2026-04-29T01:00:01Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/180952024-11-28T15:46:57Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
López-Gómez, Carlos
author
Santiago-Fernández, Concepción
author
García-Serrano, Sara
author
García-Escobar, Eva
author
Gutiérrez-Repiso, Carolina
author
Rodríguez-Díaz, Cristina
author
Ho-Plágaro, Ailec
author
Martín-Reyes, Flores
author
Garrido-Sánchez, Lourdes
author
Valdés, Sergio
author
Rodríguez-Cañete, Alberto
author
Rodríguez-Pacheco, Francisca
author
García-Fuentes, Eduardo
authoraffiliation
[López-Gómez,C; Santiago-Fernández,C; Rodríguez-Díaz,C; Ho-Plágaro,A; Martín-Reyes,F; Rodríguez-Pacheco,F; García-Fuentes,E] Unidad de Gestión Clínica de Aparato Digestivo, Hospital Universitario Virgen de la Victoria/Instituto de Investigación Biomédica de Málaga-IBIMA, Málaga, Spain. [García-Serrano,S; García-Escobar,E; Valdés,S] Unidad de Gestión Clínica de Endocrinología y Nutrición, Hospital Regional Universitario de Málaga/Instituto de Investigación Biomédica de Málaga-IBIMA, Málaga, Spain. [García-Serrano,S; García-Escobar,E; Valdés,S; Rodríguez-Pacheco,F] CIBER de Diabetes y Enfermedades Metabólicas Asociadas-CIBERDEM, Málaga, Spain. [Gutiérrez-Repiso,C; Garrido-Sánchez,L] Unidad de Gestión Clínica de Endocrinología y Nutrición, Hospital Universitario Virgen de la Victoria/Instituto de Investigación Biomédica de Málaga-IBIMA, Málaga, Spain. [Garrido-Sánchez,L] CIBER Fisiopatología de la Obesidad y Nutrición-CIBEROBN, Málaga, Spain. [Rodríguez-Cañete,A] Unidad de Gestión Clínica de Cirugía General, Digestiva y Trasplantes, Hospital Regional Universitario de Málaga, Málaga, Spain.
2024-02-12T19:46:58Z
2024-02-12T19:46:58Z
2020-08-12
http://hdl.handle.net/10668/4103
http://hdl.handle.net/20.500.12105/18095
32806641
10.3390/jcm9082615
2077-0383
Journal of Clinical Medicine
The effects of different types of fatty acids on the gene expression of key players in the IRS1/PI3K signaling pathway have been poorly studied. Material and Methods: We analyzed IRS1, p85α, and p110β mRNA expression and the fatty acid composition of phospholipids in visceral adipose tissue from patients with morbid obesity and from non-obese patients. Moreover, we analyzed the expression of those genes in visceral adipocytes incubated with oleic, linoleic, palmitic and dosahexaenoic acids. Results: We found a reduced IRS1 expression in patients with morbid obesity, independent of insulin resistance, and a reduced p110β expression in those with lower insulin resistance. A positive correlation was found between p85α and stearic acid, and between IRS1 and p110β with palmitic and dosahexaenoic acid. In contrast, a negative correlation was found between p85α and oleic acid, and between IRS1 and p110β with linoleic, arachidonic and adrenic acid. Incubation with palmitic acid decreased IRS1 expression. p85α was down-regulated after incubation with oleic and dosahexaenoic acid and up-regulated with palmitic acid. p110β expression was increased and decreased after incubation with oleic and palmitic acid, respectively. The ratio p85α/p110β was decreased by oleic and dosahexaenoic acid and increased by palmitic acid. Conclusions: Our in vitro results suggest a detrimental role of palmitic acid on the expression of gene related to insulin signaling pathway, with oleic acid being the one with the higher and more beneficial effects. DHA had a slight beneficial effect. Fatty acid-induced regulation of genes related to the IRS1/PI3K pathway may be a novel mechanism by which fatty acids regulate insulin sensitivity in visceral adipocytes.
eng
Morbid obesity
Insulin resistance
IRS1
p85α
p110β
Palmitic acid
Oleic acid
Docosahexaenoic acid
Linoleic acid
Visceral adipose tissue
Obesidad mórbida
Resistencia a la insulina
Ácido palmítico
Ácido oléico
Ácidos docosahexaenoicos
Ácido linoleico
Grasa intraabdominal
Andalucía
Oleic Acid Protects Against Insulin Resistance by Regulating the Genes Related to the PI3K Signaling Pathway
research article