2026-04-29T00:59:46Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/180952024-11-28T15:46:57Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc López-Gómez, Carlos author Santiago-Fernández, Concepción author García-Serrano, Sara author García-Escobar, Eva author Gutiérrez-Repiso, Carolina author Rodríguez-Díaz, Cristina author Ho-Plágaro, Ailec author Martín-Reyes, Flores author Garrido-Sánchez, Lourdes author Valdés, Sergio author Rodríguez-Cañete, Alberto author Rodríguez-Pacheco, Francisca author García-Fuentes, Eduardo author 2020-08-12 The effects of different types of fatty acids on the gene expression of key players in the IRS1/PI3K signaling pathway have been poorly studied. Material and Methods: We analyzed IRS1, p85α, and p110β mRNA expression and the fatty acid composition of phospholipids in visceral adipose tissue from patients with morbid obesity and from non-obese patients. Moreover, we analyzed the expression of those genes in visceral adipocytes incubated with oleic, linoleic, palmitic and dosahexaenoic acids. Results: We found a reduced IRS1 expression in patients with morbid obesity, independent of insulin resistance, and a reduced p110β expression in those with lower insulin resistance. A positive correlation was found between p85α and stearic acid, and between IRS1 and p110β with palmitic and dosahexaenoic acid. In contrast, a negative correlation was found between p85α and oleic acid, and between IRS1 and p110β with linoleic, arachidonic and adrenic acid. Incubation with palmitic acid decreased IRS1 expression. p85α was down-regulated after incubation with oleic and dosahexaenoic acid and up-regulated with palmitic acid. p110β expression was increased and decreased after incubation with oleic and palmitic acid, respectively. The ratio p85α/p110β was decreased by oleic and dosahexaenoic acid and increased by palmitic acid. Conclusions: Our in vitro results suggest a detrimental role of palmitic acid on the expression of gene related to insulin signaling pathway, with oleic acid being the one with the higher and more beneficial effects. DHA had a slight beneficial effect. Fatty acid-induced regulation of genes related to the IRS1/PI3K pathway may be a novel mechanism by which fatty acids regulate insulin sensitivity in visceral adipocytes. http://hdl.handle.net/10668/4103 http://hdl.handle.net/20.500.12105/18095 32806641 10.3390/jcm9082615 2077-0383 Journal of Clinical Medicine Morbid obesity Insulin resistance IRS1 p85α p110β Palmitic acid Oleic acid Docosahexaenoic acid Linoleic acid Visceral adipose tissue Obesidad mórbida Resistencia a la insulina Ácido palmítico Ácido oléico Ácidos docosahexaenoicos Ácido linoleico Grasa intraabdominal Andalucía Oleic Acid Protects Against Insulin Resistance by Regulating the Genes Related to the PI3K Signaling Pathway