2026-04-29T12:23:43Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/180942024-11-28T14:52:02Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Crovace, Alberto Maria
author
Lacitignola, Luca
author
Forleo, Donato Monopoli
author
Staffieri, Francesco
author
Francioso, Edda
author
Di Meo, Antonio
author
Becerra, José
author
Crovace, Antonio
author
Santos-Ruiz, Leonor
authoraffiliation
[Crovace,AM; Di Meo,A] Dottorato di Ricerca in Sanità e Scienze Sperimentali Veterinarie—DMV, University of Perugia, Perugia, Italy. [Lacitignola,L; Staffieri,F; Francioso,E; Crovace,A] Department of Emergencies and Organ Transplantation (DEOT), Valenzano (BA), Italy. [Forleo,DM] Departamento de Ingeniería Mecánica, Instituto Tecnológico de Canarias (ITC), Arinaga (Agüimes), Las Palmas de Gran Canaria, Spain. [Becerra,J; Santos-Ruiz,L] Departamento de Biología Celular, Genética y Fisiología, Facultad de Ciencias, Campus de Teatinos, Málaga, Spain. [Becerra,J; Santos-Ruiz,L] Centro Andaluz de Nanomedicina y Biotecnología (BIONAND), Parque Tecnológico de Andalucía, Campanillas-Málaga, Spain. [Becerra,J; Santos-Ruiz,L] Centro de Investigación Biomédica en Red—Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Instituto de Salud Carlos III, Madrid, Spain. [Becerra,J; Santos-Ruiz,L] Instituto de Investigación Biomédica de Málaga—IBIMA, Málaga, Spain.
2024-02-12T19:46:57Z
2024-02-12T19:46:57Z
2020-08-11
10.3390/ani10081389
2076-2615
Animals
http://hdl.handle.net/10668/4539
32796533
http://hdl.handle.net/20.500.12105/18094
The main goal in the treatment of large bone defects is to guarantee a rapid loading of the affected limb. In this paper, the authors proposed a new reconstructive technique that proved to be suitable to reach this purpose through the use of a custom-made biomimetic porous titanium scaffold. An in vivo study was undertaken where a complete critical defect was experimentally created in the diaphysis of the right tibia of twelve sheep and replaced with a five-centimeter porous scaffold of electron beam melting (EBM)-sintered titanium alloy (EBM group n = 6) or a porous hydroxyapatite scaffold (CONTROL group, n = 6). After surgery, the sheep were allowed to move freely in the barns. The outcome was monitored for up to 12 months by periodical X-ray and clinical examination. All animals in the CONTROL group were euthanized for humane reasons within the first month after surgery due to the onset of plate bending due to mechanical overload. Nine months after surgery, X-ray imaging showed the complete integration of the titanium implant in the tibia diaphysis and remodeling of the periosteal callus, with a well-defined cortical bone. At 12 months, sheep were euthanized, and the tibia were harvested and subjected to histological analysis. This showed bone tissue formations with bone trabeculae bridging titanium trabeculae, evidencing an optimal tissue-metal interaction. Our results show that EBM-sintered titanium devices, if used to repair critical bone defects in a large animal model, can guarantee immediate body weight-bearing, a rapid functional recovery, and a good osseointegration. The porous hydroxyapatite scaffolds proved to be not suitable in this model of large bone defect due to their known poor mechanical properties.
eng
Bone
Bone fracture
Bone repair
Metal endo-implant
Titanium alloy
Additive manufacturing
EBM (electron beam melting)
Biomechanics
Critical-size bone defect
Sheep
Animal model
Osseointegration
Huesos
Fracturas óseas
Titanio
Biomecánica
Ovinos
Modelos animales
Oseointegración
Prótesis e implantes
3D Biomimetic Porous Titanium (Ti6Al4V ELI) Scaffolds for Large Bone Critical Defect Reconstruction: An Experimental Study in Sheep
research article