2026-07-24T06:29:04Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/180842024-11-28T14:45:41Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Lopez-Caraballo, Lidia
author
Martorell-Marugan, Jordi
author
Carmona-Sáez, Pedro
author
Gonzalez-Munoz, Elena
authoraffiliation
[Lopez-Caraballo,L; Gonzalez-Munoz,E] Laboratory of Cell Reprogramming (LARCEL), Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, Málaga, Spain. [Martorell-Marugan,J; Carmona-Sáez,P] Bioinformatics Unit. GENYO, Centre for Genomics and Oncological Research: Pfizer/University of Granada/Andalusian Regional Government, Granada, Spain. [Martorell-Marugan,J] Atrys Health, Barcelona, Spain. [Carmona-Sáez,P] Department of Statistics. University of Granada, Granada, Spain. [Gonzalez-Munoz,E] Department of Cell Biology, Genetics and Physiology, University of Málaga, Málaga, Spain. [Gonzalez-Munoz,E] Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, (CIBER-BBN), Málaga, Spain.
2024-02-12T19:46:49Z
2024-02-12T19:46:49Z
2020-07-29
10.3390/cells9081803
2073-4409
Cells
http://hdl.handle.net/10668/3558
32751289
http://hdl.handle.net/20.500.12105/18084
The most challenging aspect of secondary progressive multiple sclerosis (SPMS) is the lack of efficient regenerative response for remyelination, which is carried out by the endogenous population of adult oligoprogenitor cells (OPCs) after proper activation. OPCs must proliferate and migrate to the lesion and then differentiate into mature oligodendrocytes. To investigate the OPC cellular component in SPMS, we developed induced pluripotent stem cells (iPSCs) from SPMS-affected donors and age-matched controls (CT). We confirmed their efficient and similar OPC differentiation capacity, although we reported SPMS-OPCs were transcriptionally distinguishable from their CT counterparts. Analysis of OPC-generated conditioned media (CM) also evinced differences in protein secretion. We further confirmed SPMS-OPC CM presented a deficient capacity to stimulate OPC in vitro migration that can be compensated by exogenous addition of specific components. Our results provide an SPMS-OPC cellular model and encouraging venues to study potential cell communication deficiencies in the progressive form of multiple sclerosis (MS) for future treatment strategies.
eng
Secondary progressive multiple sclerosis (SPMS)
Disease modeling
iPSCs
Oligodendrocyte progenitor cells (OPCs)
Secretome
Cell migration
Esclerosis múltiple crónica progresiva
Células precursoras de oligodendrocitos
Movimiento celular
Remielinización
iPS-Derived Early Oligodendrocyte Progenitor Cells from SPMS Patients Reveal Deficient In Vitro Cell Migration Stimulation
research article