2026-06-14T03:28:58Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/180822024-11-28T15:25:03Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Casanellas, Ignasi
author
Lagunas, Anna
author
Vida, Yolanda
author
Pérez-Inestrosa, Ezequiel
author
Andrades, José A.
author
Becerra, José
author
Samitier, Josep
authoraffiliation
[Casanellas,I; Lagunas,L; Samitier,J] Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology (BIST), Barcelona, Spain. [Casanellas,I; Samitier,J] Department of Electronics and Biomedical Engineering, University of Barcelona (UB), Barcelona, Spain. [Casanellas,I; Lagunas,L; Andrades,JA; Samitier,J; Becerra,J; Samitier,J] Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Madrid, Spain. [Vida,Y; Pérez-Inestrosa,E] Departamento de Química Orgánica, Universidad de Málaga-IBIMA, Málaga, Spain. [Vida,Y; Pérez-Inestrosa,E; Becerra,J] Centro Andaluz de Nanomedicina y Biotecnología-BIONAND, Campanillas, Málaga, Spain. [Andrades,JA; Becerra,J] Department of Cell Biology, Genetics and Physiology, Universidad de Málaga-IBIMA, Málaga, Spain.
2024-02-12T19:46:47Z
2024-02-12T19:46:47Z
2020-07-24
10.3390/ijms21155269
1422-0067
International Journal of Molecular Sciences
http://hdl.handle.net/10668/3581
32722300
http://hdl.handle.net/20.500.12105/18082
Tackling the first stages of the chondrogenic commitment is essential to drive chondrogenic differentiation to healthy hyaline cartilage and minimize hypertrophy. During chondrogenesis, the extracellular matrix continuously evolves, adapting to the tissue adhesive requirements at each stage. Here, we take advantage of previously developed nanopatterns, in which local surface adhesiveness can be precisely tuned, to investigate its effects on prechondrogenic condensation. Fluorescence live cell imaging, immunostaining, confocal microscopy and PCR analysis are used to follow the condensation process on the nanopatterns. Cell tracking parameters, condensate morphology, cell-cell interactions, mechanotransduction and chondrogenic commitment are evaluated in response to local surface adhesiveness. Results show that only condensates on the nanopatterns of high local surface adhesiveness are stable in culture and able to enter the chondrogenic pathway, thus highlighting the importance of controlling cell-substrate adhesion in the tissue engineering strategies for cartilage repair.
eng
Dendrimer
Nanopatterning
RGD
Mesenchymal cell condensation
Cell–cell interactions
YAP
Chondrogenesis
Dendrímeros
Comunicación celular
Condrogénesis
The Janus Role of Adhesion in Chondrogenesis
research article