2026-06-14T03:30:12Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/180822024-11-28T15:25:03Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Casanellas, Ignasi author Lagunas, Anna author Vida, Yolanda author Pérez-Inestrosa, Ezequiel author Andrades, José A. author Becerra, José author Samitier, Josep author 2020-07-24 Tackling the first stages of the chondrogenic commitment is essential to drive chondrogenic differentiation to healthy hyaline cartilage and minimize hypertrophy. During chondrogenesis, the extracellular matrix continuously evolves, adapting to the tissue adhesive requirements at each stage. Here, we take advantage of previously developed nanopatterns, in which local surface adhesiveness can be precisely tuned, to investigate its effects on prechondrogenic condensation. Fluorescence live cell imaging, immunostaining, confocal microscopy and PCR analysis are used to follow the condensation process on the nanopatterns. Cell tracking parameters, condensate morphology, cell-cell interactions, mechanotransduction and chondrogenic commitment are evaluated in response to local surface adhesiveness. Results show that only condensates on the nanopatterns of high local surface adhesiveness are stable in culture and able to enter the chondrogenic pathway, thus highlighting the importance of controlling cell-substrate adhesion in the tissue engineering strategies for cartilage repair. 10.3390/ijms21155269 1422-0067 International Journal of Molecular Sciences http://hdl.handle.net/10668/3581 32722300 http://hdl.handle.net/20.500.12105/18082 Dendrimer Nanopatterning RGD Mesenchymal cell condensation Cell–cell interactions YAP Chondrogenesis Dendrímeros Comunicación celular Condrogénesis The Janus Role of Adhesion in Chondrogenesis