2026-07-21T07:57:58Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/180362024-09-21T19:12:24Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Vonhögen, Indira G C
author
El Azzouzi, Hamid
author
Olieslagers, Servé
author
Vasilevich, Aliaksei
author
de Boer, Jan
author
Tinahones, Francisco J
author
da Costa Martins, Paula A
author
de Windt, Leon J
author
Murri, Mora
2024-02-12T19:46:01Z
2024-02-12T19:46:01Z
2020-04-23
10.3390/cells9041056
2073-4409
Cells
http://hdl.handle.net/10668/15447
32340411
http://hdl.handle.net/20.500.12105/18036
The prevalence of metabolic syndrome (MetS) and obesity is an alarming health issue worldwide. Obesity is characterized by an excessive accumulation of white adipose tissue (WAT), and it is associated with diminished brown adipose tissue (BAT) activity. Twist1 acts as a negative feedback regulator of BAT metabolism. Therefore, targeting Twist1 could become a strategy for obesity and metabolic disease. Here, we have identified miR-337-3p as an upstream regulator of Twist1. Increased miR-337-3p expression paralleled decreased expression of TWIST1 in BAT compared to WAT. Overexpression of miR-337-3p in brown pre-adipocytes provoked a reduction in Twist1 expression that was accompanied by increased expression of brown/mitochondrial markers. Luciferase assays confirmed an interaction between the miR-337 seed sequence and Twist1 3'UTR. The inverse relationship between the expression of TWIST1 and miR-337 was finally validated in adipose tissue samples from non-MetS and MetS subjects that demonstrated a dysregulation of the miR-337-Twist1 molecular axis in MetS. The present study demonstrates that adipocyte miR-337-3p suppresses Twist1 repression and enhances the browning of adipocytes.
eng
Brown adipose tissue
Metabolic syndrome
microRNA
Mitochondria
Obesity
MiR-337-3p Promotes Adipocyte Browning by Inhibiting TWIST1.
research article