2026-07-21T07:57:57Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/180362024-09-21T19:12:24Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Vonhögen, Indira G C author El Azzouzi, Hamid author Olieslagers, Servé author Vasilevich, Aliaksei author de Boer, Jan author Tinahones, Francisco J author da Costa Martins, Paula A author de Windt, Leon J author Murri, Mora author 2020-04-23 The prevalence of metabolic syndrome (MetS) and obesity is an alarming health issue worldwide. Obesity is characterized by an excessive accumulation of white adipose tissue (WAT), and it is associated with diminished brown adipose tissue (BAT) activity. Twist1 acts as a negative feedback regulator of BAT metabolism. Therefore, targeting Twist1 could become a strategy for obesity and metabolic disease. Here, we have identified miR-337-3p as an upstream regulator of Twist1. Increased miR-337-3p expression paralleled decreased expression of TWIST1 in BAT compared to WAT. Overexpression of miR-337-3p in brown pre-adipocytes provoked a reduction in Twist1 expression that was accompanied by increased expression of brown/mitochondrial markers. Luciferase assays confirmed an interaction between the miR-337 seed sequence and Twist1 3'UTR. The inverse relationship between the expression of TWIST1 and miR-337 was finally validated in adipose tissue samples from non-MetS and MetS subjects that demonstrated a dysregulation of the miR-337-Twist1 molecular axis in MetS. The present study demonstrates that adipocyte miR-337-3p suppresses Twist1 repression and enhances the browning of adipocytes. 10.3390/cells9041056 2073-4409 Cells http://hdl.handle.net/10668/15447 32340411 http://hdl.handle.net/20.500.12105/18036 Brown adipose tissue Metabolic syndrome microRNA Mitochondria Obesity MiR-337-3p Promotes Adipocyte Browning by Inhibiting TWIST1.