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oai:repisalud.isciii.es:20.500.12105/179892024-11-28T15:32:08Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud author Caro-Astorga, Joaquín author Frenzel, Elrike author Perkins, James R. author Álvarez-Mena, Ana author de Vicente, Antonio author Ranea, Juan A. G. author Kuipers, Oscar P. author Romero, Diego authoraffiliation [Caro-Astorga,J; Álvarez-Mena,A; de Vicente,A; Romero,D] Departamento de Microbiología, Universidad de Málaga, Málaga, Spain. [Frenzel,E; Kuipers,OP] Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen, Groningen, The Netherlands. [Perkins,JR] Research Laboratory, IBIMA, Regional University Hospital of Malaga-UMA, Málaga, Spain. [Perkins,JR; Ranea,JAG] CIBER de Enfermedades Raras (CIBERER), ISCIII Madrid, Spain. [Ranea,JAG] Department of Molecular Biology and Biochemistry, Universidad de Málaga, Málaga, Spain. 2024-02-12T19:45:09Z 2024-02-12T19:45:09Z 2020-01-15 http://hdl.handle.net/10668/4026 http://hdl.handle.net/20.500.12105/17989 31969984 10.1038/s41522-019-0112-7 2055-5008 NPJ Biofilms and Microbiomes Biofilm formation is a strategy of many bacterial species to adapt to a variety of stresses and has become a part of infections, contaminations, or beneficial interactions. In this study, we demonstrate that profound physiological changes permit Bacillus cereus to switch from a floating to a sessile lifestyle, to undergo further maturation of the biofilm and to differentiate into the offensive or defensive features. We report that floating and biofilm cells are populations that differentiate metabolically, with members of each subpopulation developing different branches of certain metabolic pathways. Secondly, biofilm populations rearrange nucleotides, sugars, amino acids, and energy metabolism. Thirdly, this metabolic rearrangement coexists with: the synthesis of the extracellular matrix, sporulation, reinforcement of the cell wall, activation of the ROS detoxification machinery and production of secondary metabolites. This strategy contributes to defend biofilm cells from competitors. However, floating cells maintain a fermentative metabolic status that ensures a higher aggressiveness against hosts, evidenced by the production of toxins. The maintenance of the two distinct subpopulations is an effective strategy to face different environmental conditions found in the life styles of B. cereus. eng Biofilms Next-generation sequencing Bacillus cereus Biopelículas Secuenciación de nucleótidos de alto rendimiento Biofilm formation displays intrinsic offensive and defensive features of Bacillus cereus research article