2026-07-22T08:07:36Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/179842024-11-28T15:27:37Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927col_20.500.12105_16958col_20.500.12105_16962

00925njm 22002777a 4500 dc Fernández-Muñoz, Beatriz author Rosell-Valle, Cristina author Ferrari, Daniela author Alba-Amador, Julia author Montiel, Miguel Ángel author Campos-Cuerva, Rafael author Lopez-Navas, Luis author Muñoz-Escalona, María author Martín-López, María author Profico, Daniela Celeste author Blanco, Manuel Francisco author Giorgetti, Alessandra author González-Muñoz, Elena author Márquez-Rivas, Javier author Sanchez-Pernaute, Rosario author 2020 Intraventricular hemorrhage is a common cause of morbidity and mortality in premature infants. The rupture of the germinal zone into the ventricles entails loss of neural stem cells and disturbs the normal cytoarchitecture of the region, compromising late neurogliogenesis. Here we demonstrate that neural stem cells can be easily and robustly isolated from the hemorrhagic cerebrospinal fluid obtained during therapeutic neuroendoscopic lavage in preterm infants with severe intraventricular hemorrhage. Our analyses demonstrate that these neural stem cells, although similar to human fetal cell lines, display distinctive hallmarks related to their regional and developmental origin in the germinal zone of the ventral forebrain, the ganglionic eminences that give rise to interneurons and oligodendrocytes. These cells can be expanded, cryopreserved, and differentiated in vitro and in vivo in the brain of nude mice and show no sign of tumoral transformation 6 months after transplantation. This novel class of neural stem cells poses no ethical concerns, as the fluid is usually discarded, and could be useful for the development of an autologous therapy for preterm infants, aiming to restore late neurogliogenesis and attenuate neurocognitive deficits. Furthermore, these cells represent a valuable tool for the study of the final stages of human brain development and germinal zone biology. 10.1002/sctm.19-0323 2157-6580 2157-6564 Stem Cells Translational Medicine http://hdl.handle.net/10668/3868 32475061 http://hdl.handle.net/20.500.12105/17984 Cerebrospinal fluid Germinal zone Intraventricular hemorrhage Neural stem cell Neurogenesis Premature infant Líquido cefalorraquídeo Centro germinal Hemorragia cerebral intraventricular Células madre nerviosas Recién nacido prematuro Retrieval of germinal zone neural stem cells from the cerebrospinal fluid of premature infants with intraventricular hemorrhage