2026-06-14T03:44:27Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/179522024-11-29T18:46:59Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Bernad, Rafael
author
Sánchez, Patricia
author
Rivera, Teresa
author
Rodríguez-Corsino, Miriam
author
Boyarchuk, Ekaterina
author
Vassias, Isabelle
author
Ray-Gallet, Dominique
author
Arnaoutov, Alexei
author
Dasso, Mary
author
Almouzni, Geneviève
author
Losada, Ana
funder
Unión Europea. Comisión Europea
funder
Ministerio de Ciencia (España)
funder
Unión Europea
funder
Agence Nationale de la Recherche (Francia)
funder
Breast cancer and Epigenetics
2024-02-12T06:21:24Z
2024-02-12T06:21:24Z
2011-02-21
J Cell Biol . 2011 ;192(4):569-82.
10.1083/jcb.201005136
1540-8140
The Journal of cell biology
21321101
http://hdl.handle.net/20.500.12105/17952
Centromeric protein A (CENP-A) is the epigenetic mark of centromeres. CENP-A replenishment is necessary in each cell cycle to compensate for the dilution associated to DNA replication, but how this is achieved mechanistically is largely unknown. We have developed an assay using Xenopus egg extracts that can recapitulate the spatial and temporal specificity of CENP-A deposition observed in human cells, providing us with a robust in vitro system amenable to molecular dissection. Here we show that this deposition depends on Xenopus Holliday junction-recognizing protein (xHJURP), a member of the HJURP/Scm3 family recently identified in yeast and human cells, further supporting the essential role of these chaperones in CENP-A loading. Despite little sequence homology, human HJURP can substitute for xHJURP. We also report that condensin II, but not condensin I, is required for CENP-A assembly and contributes to retention of centromeric CENP-A nucleosomes both in mitosis and interphase. We propose that the chromatin structure imposed by condensin II at centromeres enables CENP-A incorporation initiated by xHJURP.
eng
Xenopus HJURP and condensin II are required for CENP-A assembly.
journal article
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URL
https://repisalud.isciii.es/bitstreams/c1b67f1f-354f-4fd1-b8e4-62a4d3c42e91/download
File
MD5
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application/pdf
XenopusHJURP_2011.pdf
URL
https://repisalud.isciii.es/bitstreams/2d58f123-49a1-4a59-a053-1f715b5545f0/download
File
MD5
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text/plain
XenopusHJURP_2011.pdf.txt