2026-07-22T20:05:50Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/178782024-09-21T20:46:50Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927col_20.500.12105_16988
Repisalud
author
Mantilla-Escalante, Diana C
author
López de Las Hazas, María-Carmen
author
Gil-Zamorano, Judit
author
Del Pozo-Acebo, Lorena
author
Crespo, M Carmen
author
Martín-Hernández, Roberto
author
Del Saz, Andrea
author
Tomé-Carneiro, Joao
author
Cardona, Fernando
author
Cornejo-Pareja, Isabel
author
García-Ruiz, Almudena
author
Briand, Olivier
author
Lasunción, Miguel A
author
Visioli, Francesco
author
Dávalos, Alberto
2024-02-10T20:01:41Z
2024-02-10T20:01:41Z
2019-06-13
10.3390/nu11061326
2072-6643
Nutrients
http://hdl.handle.net/10668/14116
31200481
http://hdl.handle.net/20.500.12105/17878
Postprandial lipemia has many physiopathological effects, some of which increase the risk of cardiovascular disease. MicroRNAs (miRNAs) can be found in almost all biological fluids, but their postprandial kinetics are poorly described. We aimed to profile circulating miRNAs in response to a fat challenge. In total, 641 circulating miRNAs were assessed by real-time PCR in plasmas from mice two hours after lipid gavage. Mice with intestine-specific loss of Dicer were screened to identify potential miRNAs released by the intestine. A total of 68 miRNAs were selected for further validation. Ten circulating miRNAs were finally validated as responsive to postprandial lipemia, including miR-206-3p, miR-543-3p, miR-466c-5p, miR-27b-5p, miR-409-3p, miR-340-3p, miR-1941-3p, miR-10a-3p, miR-125a-3p, and miR-468-3p. Analysis of their possible tissues of origin/target showed an enrichment of selected miRNAs in liver, intestine, brain, or skeletal muscle. miR-206, miR-27b-5p, and miR-409-3p were validated in healthy humans. Analysis of their predicted target genes revealed their potential involvement in insulin/insulin like growth factor (insulin/IGF), angiogenesis, cholecystokinin B receptor signaling pathway (CCKR), inflammation or Wnt pathways for mice, and in platelet derived growth factor (PDGF) and CCKR signaling pathways for humans. Therefore, the current study shows that certain miRNAs are released in the circulation in response to fatty meals, proposing them as potential novel therapeutic targets of lipid metabolism.
eng
Circulating miRNA
Diet
Exosomes
Fat challenge
Postprandial
Postprandial Circulating miRNAs in Response to a Dietary Fat Challenge.
research article