2026-07-22T20:10:59Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/178782024-09-21T20:46:50Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927col_20.500.12105_16988

00925njm 22002777a 4500 dc Mantilla-Escalante, Diana C author López de Las Hazas, María-Carmen author Gil-Zamorano, Judit author Del Pozo-Acebo, Lorena author Crespo, M Carmen author Martín-Hernández, Roberto author Del Saz, Andrea author Tomé-Carneiro, Joao author Cardona, Fernando author Cornejo-Pareja, Isabel author García-Ruiz, Almudena author Briand, Olivier author Lasunción, Miguel A author Visioli, Francesco author Dávalos, Alberto author 2019-06-13 Postprandial lipemia has many physiopathological effects, some of which increase the risk of cardiovascular disease. MicroRNAs (miRNAs) can be found in almost all biological fluids, but their postprandial kinetics are poorly described. We aimed to profile circulating miRNAs in response to a fat challenge. In total, 641 circulating miRNAs were assessed by real-time PCR in plasmas from mice two hours after lipid gavage. Mice with intestine-specific loss of Dicer were screened to identify potential miRNAs released by the intestine. A total of 68 miRNAs were selected for further validation. Ten circulating miRNAs were finally validated as responsive to postprandial lipemia, including miR-206-3p, miR-543-3p, miR-466c-5p, miR-27b-5p, miR-409-3p, miR-340-3p, miR-1941-3p, miR-10a-3p, miR-125a-3p, and miR-468-3p. Analysis of their possible tissues of origin/target showed an enrichment of selected miRNAs in liver, intestine, brain, or skeletal muscle. miR-206, miR-27b-5p, and miR-409-3p were validated in healthy humans. Analysis of their predicted target genes revealed their potential involvement in insulin/insulin like growth factor (insulin/IGF), angiogenesis, cholecystokinin B receptor signaling pathway (CCKR), inflammation or Wnt pathways for mice, and in platelet derived growth factor (PDGF) and CCKR signaling pathways for humans. Therefore, the current study shows that certain miRNAs are released in the circulation in response to fatty meals, proposing them as potential novel therapeutic targets of lipid metabolism. 10.3390/nu11061326 2072-6643 Nutrients http://hdl.handle.net/10668/14116 31200481 http://hdl.handle.net/20.500.12105/17878 Circulating miRNA Diet Exosomes Fat challenge Postprandial Postprandial Circulating miRNAs in Response to a Dietary Fat Challenge.