2026-07-21T16:28:22Zhttps://repisalud.isciii.es/rest/oai/request
oai:repisalud.isciii.es:20.500.12105/178552024-09-21T20:35:59Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud author Molina-Santiago, Carlos author Pearson, John R author Navarro, Yurena author Berlanga-Clavero, María Victoria author Caraballo-Rodriguez, Andrés Mauricio author Petras, Daniel author García-Martín, María Luisa author Lamon, Gaelle author Haberstein, Birgit author Cazorla, Francisco M author de Vicente, Antonio author Loquet, Antoine author Dorrestein, Pieter C author Romero, Diego 2024-02-10T20:01:21Z 2024-02-10T20:01:21Z 2019-04-23 10.1038/s41467-019-09944-x 2041-1723 Nature communications http://hdl.handle.net/10668/13871 31015472 http://hdl.handle.net/20.500.12105/17855 Bacteria of the genera Pseudomonas and Bacillus can promote plant growth and protect plants from pathogens. However, the interactions between these plant-beneficial bacteria are understudied. Here, we explore the interaction between Bacillus subtilis 3610 and Pseudomonas chlororaphis PCL1606. We show that the extracellular matrix protects B. subtilis colonies from infiltration by P. chlororaphis. The absence of extracellular matrix results in increased fluidity and loss of structure of the B. subtilis colony. The P. chlororaphis type VI secretion system (T6SS) is activated upon contact with B. subtilis cells, and stimulates B. subtilis sporulation. Furthermore, we find that B. subtilis sporulation observed prior to direct contact with P. chlororaphis is mediated by histidine kinases KinA and KinB. Finally, we demonstrate the importance of the extracellular matrix and the T6SS in modulating the coexistence of the two species on melon plant leaves and seeds. eng The extracellular matrix protects Bacillus subtilis colonies from Pseudomonas invasion and modulates plant co-colonization. research article