2026-05-16T22:10:10Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/178172025-05-07T09:06:46Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Caro, Carlos
author
Quaresma, Pedro
author
Pereira, Eulália
author
Franco, Jaime
author
Pernia Leal, Manuel
author
García-Martín, Maria Luisa
author
Royo, José Luis
author
Oliva-Montero, Jose Maria
author
Merkling, Patrick Jacques
author
Zaderenko, Ana Paula
author
Pozo, David
author
Franco, Ricardo
2024-02-10T20:00:46Z
2024-02-10T20:00:46Z
2019-02-13
10.3390/nano9020256
2079-4991
Nanomaterials (Basel, Switzerland)
http://hdl.handle.net/10668/13590
30781838
http://hdl.handle.net/20.500.12105/17817
Progress in the field of biocompatible SERS nanoparticles has promising prospects for biomedical applications. In this work, we have developed a biocompatible Raman probe by combining anisotropic silver nanoparticles with the dye rhodamine 6G followed by subsequent coating with bovine serum albumin. This nanosystem presents strong SERS capabilities in the near infrared (NIR) with a very high (2.7 × 10⁷) analytical enhancement factor. Theoretical calculations reveal the effects of the electromagnetic and chemical mechanisms in the observed SERS effect for this nanosystem. Finite element method (FEM) calculations showed a considerable near field enhancement in NIR. Using density functional quantum chemical calculations, the chemical enhancement mechanism of rhodamine 6G by interaction with the nanoparticles was probed, allowing us to calculate spectra that closely reproduce the experimental results. The nanosystem was tested in cell culture experiments, showing cell internalization and also proving to be completely biocompatible, as no cell death was observed. Using a NIR laser, SERS signals could be detected even from inside cells, proving the applicability of this nanosystem as a biocompatible SERS probe.
eng
SERS
Cancer
Cell labeling
Density functional theory calculations
finite element method
surface enhanced Raman scattering
Synthesis and Characterization of Elongated-Shaped Silver Nanoparticles as a Biocompatible Anisotropic SERS Probe for Intracellular Imaging: Theoretical Modeling and Experimental Verification.
research article