2026-05-16T22:10:09Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/178172025-05-07T09:06:46Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Caro, Carlos author Quaresma, Pedro author Pereira, Eulália author Franco, Jaime author Pernia Leal, Manuel author García-Martín, Maria Luisa author Royo, José Luis author Oliva-Montero, Jose Maria author Merkling, Patrick Jacques author Zaderenko, Ana Paula author Pozo, David author Franco, Ricardo author 2019-02-13 Progress in the field of biocompatible SERS nanoparticles has promising prospects for biomedical applications. In this work, we have developed a biocompatible Raman probe by combining anisotropic silver nanoparticles with the dye rhodamine 6G followed by subsequent coating with bovine serum albumin. This nanosystem presents strong SERS capabilities in the near infrared (NIR) with a very high (2.7 × 10⁷) analytical enhancement factor. Theoretical calculations reveal the effects of the electromagnetic and chemical mechanisms in the observed SERS effect for this nanosystem. Finite element method (FEM) calculations showed a considerable near field enhancement in NIR. Using density functional quantum chemical calculations, the chemical enhancement mechanism of rhodamine 6G by interaction with the nanoparticles was probed, allowing us to calculate spectra that closely reproduce the experimental results. The nanosystem was tested in cell culture experiments, showing cell internalization and also proving to be completely biocompatible, as no cell death was observed. Using a NIR laser, SERS signals could be detected even from inside cells, proving the applicability of this nanosystem as a biocompatible SERS probe. 10.3390/nano9020256 2079-4991 Nanomaterials (Basel, Switzerland) http://hdl.handle.net/10668/13590 30781838 http://hdl.handle.net/20.500.12105/17817 SERS Cancer Cell labeling Density functional theory calculations finite element method surface enhanced Raman scattering Synthesis and Characterization of Elongated-Shaped Silver Nanoparticles as a Biocompatible Anisotropic SERS Probe for Intracellular Imaging: Theoretical Modeling and Experimental Verification.