2026-04-29T00:59:53Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/178052024-09-21T22:13:21Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud
author
Perucca Orfei, Carlotta
author
Viganò, Marco
author
Pearson, John R
author
Colombini, Alessandra
author
De Luca, Paola
author
Ragni, Enrico
author
Santos-Ruiz, Leonor
author
de Girolamo, Laura
2024-02-10T20:00:37Z
2024-02-10T20:00:37Z
2019-01-03
http://hdl.handle.net/10668/13376
http://hdl.handle.net/20.500.12105/17805
30609804
10.3390/ijms20010149
1422-0067
International journal of molecular sciences
Mesenchymal Stem Cells (MSCs) and tissue-specific progenitors have been proposed as useful tools for regenerative medicine approaches in bone, cartilage and tendon-related pathologies. The differentiation of cells towards the desired, target tissue-specific lineage has demonstrated advantages in the application of cell therapies and tissue engineering. Unlike osteogenic and chondrogenic differentiation, there is no consensus on the best tenogenic induction protocol. Many growth factors have been proposed for this purpose, including BMP-12, b-FGF, TGF-β3, CTGF, IGF-1 and ascorbic acid (AA). In this study, different combinations of these growth factors have been tested in the context of a two-step differentiation protocol, in order to define their contribution to the induction and maintenance of tendon marker expression in adipose tissue and bone marrow derived MSCs and tendon cells (TCs), respectively. Our results demonstrate that TGF-β3 is the main inducer of scleraxis, an early expressed tendon marker, while at the same time inhibiting tendon markers normally expressed later, such as decorin. In contrast, we find that decorin is induced by BMP-12, b-FGF and AA. Our results provide new insights into the effect of different factors on the tenogenic induction of MSCs and TCs, highlighting the importance of differential timing in TGF-β3 stimulation.
eng
BMP-12
Bone marrow-derived MSCs
FGF
TGFbeta
adipose-derived MSCs
ascorbic acid
tendon cells
tenogenic differentiation
In Vitro Induction of Tendon-Specific Markers in Tendon Cells, Adipose- and Bone Marrow-Derived Stem Cells is Dependent on TGFβ3, BMP-12 and Ascorbic Acid Stimulation.
research article