2026-08-12T20:37:19Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/177102025-03-20T10:54:27Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Arboleda-García, Andrés author Alarcón-Ruiz, Iván author Boada-Acosta, Lissette author Boada, Yadira author Vignoni, Alejandro author Jantus-Lewintre, Eloisa author 2023-10 Synthetic biology aims to program living bacteria cells with artificial genetic circuits for user-defined functions, transforming them into powerful tools with numerous applications in various fields, including oncology. Cancer treatments have serious side effects on patients due to the systemic action of the drugs involved. To address this, new systems that provide localized antitumoral action while minimizing damage to healthy tissues are required. Bacteria, often considered pathogenic agents, have been used as cancer treatments since the early 20th century. Advances in genetic engineering, synthetic biology, microbiology, and oncology have improved bacterial therapies, making them safer and more effective. Here we propose six modules for a successful synthetic biology-based bacterial cancer therapy, the modules include Payload, Release, Tumor-targeting, Biocontainment, Memory, and Genetic Circuit Stability Module. These will ensure antitumor activity, safety for the environment and patient, prevent bacterial colonization, maintain cell stability, and prevent loss or defunctionalization of the genetic circuit. Crit Rev Oncol Hematol. 2023 Oct:190:104088. 10.1016/j.critrevonc.2023.104088 1879-0461 Critical reviews in oncology/hematology 37541537 http://hdl.handle.net/20.500.12105/17710 Advancements in synthetic biology-based bacterial cancer therapy: A modular design approach.