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oai:repisalud.isciii.es:20.500.12105/177052024-11-29T23:28:10Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Fernandez-Capetillo, Oscar author Mahadevaiah, Shantha K author Celeste, Arkady author Romanienko, Peter J author Camerini-Otero, R Daniel author Bonner, William M author Manova, Katia author Burgoyne, Paul author Nussenzweig, André author capetillo author 2003-04 During meiotic prophase in male mammals, the X and Y chromosomes condense to form a macrochromatin body, termed the sex, or XY, body, within which X- and Y-linked genes are transcriptionally repressed. The molecular basis and biological function of both sex body formation and meiotic sex chromosome inactivation (MSCI) are unknown. A phosphorylated form of H2AX, a histone H2A variant implicated in DNA repair, accumulates in the sex body in a manner independent of meiotic recombination-associated double-strand breaks. Here we show that the X and Y chromosomes of histone H2AX-deficient spermatocytes fail to condense to form a sex body, do not initiate MSCI, and exhibit severe defects in meiotic pairing. Moreover, other sex body proteins, including macroH2A1.2 and XMR, do not preferentially localize with the sex chromosomes in the absence of H2AX. Thus, H2AX is required for the chromatin remodeling and associated silencing in male meiosis. Dev Cell . 2003;4(4):497-508. 10.1016/s1534-5807(03)00093-5 1534-5807 Developmental cell 12689589 http://hdl.handle.net/20.500.12105/17705 H2AX is required for chromatin remodeling and inactivation of sex chromosomes in male mouse meiosis.