2026-06-14T03:33:51Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/176982024-09-27T21:20:53Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Bravo-Barriga, Daniel
author
Ferraguti, Martina
author
Magallanes, Sergio
author
Aguilera-Sepúlveda, Pilar
author
Llorente, Francisco
author
Pérez-Ramírez, Elisa
author
Vazquez, Ana
author
Guerrero-Carvajal, Fátima
author
Sánchez-Seco, María Paz
author
Jiménez-Clavero, Miguel Ángel
author
Mora-Rubio, Carlos
author
Marzal, Alfonso
author
Frontera, Eva
author
de Lope, Florentino
funder
Fundación BBVA
funder
Agencia Estatal de Investigación (España)
funder
Instituto de Salud Carlos III
funder
Government of Extremadura (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Unión Europea. Comisión Europea. H2020
funder
Ministerio de Universidades (España)
2024-02-09T12:21:14Z
2024-02-09T12:21:14Z
2023
Transboundary and Emerging Diseases. 2023; 6893677.
10.1155/2023/6893677
1865-1682
1865-1674
Transboundary and Emerging Diseases
http://hdl.handle.net/20.500.12105/17698
Usutu virus (USUV) is an emerging zoonotic arbovirus that has caused an increasing number of animal and human cases in Europe in recent years. Understanding the vector species and avian hosts involved in the USUV enzootic cycle in an area of active circulation is vital to anticipate potential outbreaks. Mosquitoes were captured in 2020, while wild birds were sampled in both 2020 and 2021 in Extremadura, southwestern Spain. +e presence of USUV in the mosquito vectors was assessed by a real-time reverse transcription-polymerase chain reaction (rRT-PCR) assay and con9rmed by sequencing amplicons from two generic RTPCR sets for :aviviruses. Sequences were analysed phylogenetically. Bird sera were screened for :avivirus antibodies with a blocking ELISA kit and subsequently tested for virus-speci9c antibodies with a micro-virus-neutralization test. Overall, 6,004 mosquitoes belonging to 13 species were captured, including some well-known :avivirus vectors (Culex pipiens, Cx. perexiguus, and Cx. univittatus). Of the 438 pools tested, USUV was detected in two pools of Cx. pipiens. Phylogenetic analysis using a fragment of the NS5 gene assigned the USUV detected the Africa 3 lineage. Out of 1,413 wild birds tested, USUV-speci9c antibodies were detected in 17 birds (1.2%, 10 males and 7 females) from eight species. +e9rst detection of USUV Africa 3 lineage in mosquitoes from Spain, together with serologically positive resident wild birds in urban and rural areas, indicates active circulation and a possible risk of exposure for the human population, with necessity to establish speci9c surveillance plans.
eng
Usutu virus (USUV)
Emerging zoonotic
Extremadura (Spain)
Mosquito vectors
USUV Africa 3
Identification of Usutu Virus Africa 3 Lineage in a Survey of Mosquitoes and Birds from Urban Areas of Western Spain
research article
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