2026-06-14T03:33:47Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/176972024-09-27T19:54:42Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Sahuquillo-Arce, José Miguel author Reyes-Prieto, Mariana author Hernández-Cabezas, Alicia author Molina-Moreno, José Miguel author Saez-Nieto, Juan Antonio author Marín, María Del Pilar author Alcoriza-Balaguer, María Isabel author Lahoz, Agustín author Sanz, Jaime author González-Tarancón, Lola author Ferrús, María Loreto author Martínez-Priego, Llúcia author Magraner-Martínez, Adolfo author López-Hontangas, José Luis author 2023-08 Strain HF14-78462T is an environmental bacterium found in clinical samples from an immunocompromized patient in 2014 at Hospital Universitari i Politècnic La Fe (Valencia, Spain). Phenotypically, strain HF14-78462T cells were Gram-stain-negative, aerobic, non-spore forming and non-motile small rods which formed mucous and whitish-translucent colonies when incubated at 20-36 °C. Phylogenetic analyses based on the 16S rRNA genes and the whole genomes of closest sequenced relatives confirmed that strain HF14-78462T is affiliated with the genus Starkeya. The strain was oxidase, catalase and urease positive; but indole, lysine decarboxylase, ornithine decarboxylase and DNase negative, did not produce H2S and was able to utilize a wide variety of carbon sources including acetamide, adonitol, amygdalin, l-arabinose, citric acid, glucose, mannitol and melibiose. Unlike Starkeya novella and Starkeya koreensis, strain HF14-78462T failed to grow in thiosulphate-oxidizing media and had a narrower temperature growth range. Its genome was characterized by a size of 4.83 Mbp and a C+G content of 67.75 mol%. Major fatty acids were C18:1 ω7c, cyclo C19 : 0 and C16 : 0, its polar acids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an aminophospholipid; while the ubiquinones were Q9 (1.8 %) and Q10 (98.2 %). Digital DNA-DNA hybridization values were 41 and 41.4 against S. novella and S. koreensis, respectively, while average nucleotide identity values were around 84 %. Phenotypic, average nucleotide identity and phylogenomic comparative studies suggest that strain HF14-78462T is a new representative of the genus Starkeya and the name Starkeya nomas sp. nov. is proposed. The type strain is HF14-78462T (=CECT 30124T=LMG 31874T). Int J Syst Evol Microbiol. 2023 Aug;73(8). 10.1099/ijsem.0.005805 1466-5034 International journal of systematic and evolutionary microbiology 37605998 http://hdl.handle.net/20.500.12105/17697 Emerging pathogens Immunocompromized patient Starkeya Starkeya nomas sp. nov., a prosthecate and budding bacterium isolated from an immunocompromized patient