2026-04-28T21:35:16Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/176962025-03-20T10:48:03Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Fueyo-Marcos, Elena
author
Lopez-Pernas, Gema
author
Fustero-Torre, Coral
author
Antón, Marta Elena
author
Al-Shahrour, Fatima
author
Fernández-Capetillo, Oscar
author
Murga, Matilde
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Asociación Española Contra el Cáncer
funder
Instituto de Salud Carlos III
funder
Ministerio de Ciencia, Innovación y Universidades (España)
2024-02-09T12:00:16Z
2024-02-09T12:00:16Z
2023-03-22
Aging (Albany NY) . 2023;15(6):1791-1807.
http://hdl.handle.net/20.500.12105/17696
36947705
10.18632/aging.204598
1945-4589
Aging
Antibodies targeting the PD-1 receptor and its ligand PD-L1 have shown impressive responses in some tumors of bad prognosis. We hypothesized that, since immunosuppressive cells might present several immune checkpoints on their surface, the selective elimination of PD-L1 expressing cells could be efficacious in enabling the activation of antitumoral immune responses. To address this question, we developed an inducible suicidal knock-in mouse allele of Pd-l1 (PD-L1ATTAC) which allows for the tracking and specific elimination of PD-L1-expressing cells in adult tissues. Consistent with our hypothesis, elimination of PD-L1 expressing cells from the mouse peritoneum increased the septic response to lipopolysaccharide (LPS), due to an exacerbated inflammatory response to the endotoxin. In addition, mice depleted of PD-L1+ cells were resistant to colon cancer peritoneal allografts, which was associated with a loss of immunosuppressive B cells and macrophages, concomitant with an increase in activated cytotoxic CD8 T cells. Collectively, these results illustrate the usefulness of PD-L1ATTAC mice for research in immunotherapy and provide genetic support to the concept of targeting PD-L1 expressing cells in cancer.
eng
PD-L1ATTAC mice reveal the potential of depleting PD-L1 expressing cells in cancer therapy.
journal article
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URL
https://repisalud.isciii.es/bitstreams/9fa0dee8-74fa-4535-a806-d1e08c56a4f4/download
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PD-L1ATTAC mice reveal_2023.docx
URL
https://repisalud.isciii.es/bitstreams/d04364a1-b889-4860-ad74-0265becb5be6/download
File
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text/plain
PD-L1ATTAC mice reveal_2023.docx.txt