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oai:repisalud.isciii.es:20.500.12105/176892024-11-29T13:53:22Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Marión, Rosa M author Strati, Katerina author Li, Han author Murga, Matilde author Blanco, Raquel author Ortega Jimenez, Sagrario author Fernandez-Capetillo, Oscar author Serrano, Manuel author Blasco, MA author 2009-08-27 The reprogramming of differentiated cells to pluripotent cells (induced pluripotent stem (iPS) cells) is known to be an inefficient process. We recently reported that cells with short telomeres cannot be reprogrammed to iPS cells despite their normal proliferation rates, probably reflecting the existence of 'reprogramming barriers' that abort the reprogramming of cells with uncapped telomeres. Here we show that p53 (also known as Trp53 in mice and TP53 in humans) is critically involved in preventing the reprogramming of cells carrying various types of DNA damage, including short telomeres, DNA repair deficiencies, or exogenously inflicted DNA damage. Reprogramming in the presence of pre-existing, but tolerated, DNA damage is aborted by the activation of a DNA damage response and p53-dependent apoptosis. Abrogation of p53 allows efficient reprogramming in the face of DNA damage and the generation of iPS cells carrying persistent DNA damage and chromosomal aberrations. These observations indicate that during reprogramming cells increase their intolerance to different types of DNA damage and that p53 is critical in preventing the generation of human and mouse pluripotent cells from suboptimal parental cells. Nature . 2009 ;460(7259):1149-53. 10.1038/nature08287 1476-4687 Nature https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3624089 19668189 http://hdl.handle.net/20.500.12105/17689 A p53-mediated DNA damage response limits reprogramming to ensure iPS cell genomic integrity.