2026-08-29T20:41:05Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/176822024-11-29T15:45:02Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Lopez-Contreras, Andres J
author
Ruppen, Isabel
author
Nieto-Soler, Maria
author
Murga, Matilde
author
Rodriguez-Acebes, Sara
author
Remeseiro, Silvia
author
Rodrigo-Perez, Sara
author
Rojas, Ana M
author
Mendez, Juan
author
Muñoz, Javier
author
Fernandez-Capetillo, Oscar
funder
Asociación Española Contra el Cáncer
funder
Fundación La Caixa
funder
Ministerio de Ciencia (España)
2024-02-09T09:54:53Z
2024-02-09T09:54:53Z
2013-04-25
Cell Rep . 2013 ;3(4):1105-16.
10.1016/j.celrep.2013.03.009
2211-1247
Cell reports
23545495
http://hdl.handle.net/20.500.12105/17682
DNA replication is facilitated by multiple factors that concentrate in the vicinity of replication forks. Here, we developed an approach that combines the isolation of proteins on nascent DNA chains with mass spectrometry (iPOND-MS), allowing a comprehensive proteomic characterization of the human replisome and replisome-associated factors. In addition to known replisome components, we provide a broad list of proteins that reside in the vicinity of the replisome, some of which were not previously associated with replication. For instance, our data support a link between DNA replication and the Williams-Beuren syndrome and identify ZNF24 as a replication factor. In addition, we reveal that SUMOylation is widespread for factors that concentrate near replisomes, which contrasts with lower UQylation levels at these sites. This resource provides a panoramic view of the proteins that concentrate in the surroundings of the replisome, which should facilitate future investigations on DNA replication and genome maintenance.
eng
A proteomic characterization of factors enriched at nascent DNA molecules.
journal article
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URL
https://repisalud.isciii.es/bitstreams/21c8d6d6-25a8-4772-9549-ce0e458266ec/download
File
MD5
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application/pdf
aproteomiccharcaterization_.pdf
URL
https://repisalud.isciii.es/bitstreams/53213321-785b-4a0e-a539-a1afae9189dd/download
File
MD5
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text/plain
aproteomiccharcaterization_.pdf.txt