2026-05-21T23:03:40Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/176822024-11-29T15:45:02Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Lopez-Contreras, Andres J author Ruppen, Isabel author Nieto-Soler, Maria author Murga, Matilde author Rodriguez-Acebes, Sara author Remeseiro, Silvia author Rodrigo-Perez, Sara author Rojas, Ana M author Mendez, Juan author Muñoz, Javier author Fernandez-Capetillo, Oscar author 2013-04-25 DNA replication is facilitated by multiple factors that concentrate in the vicinity of replication forks. Here, we developed an approach that combines the isolation of proteins on nascent DNA chains with mass spectrometry (iPOND-MS), allowing a comprehensive proteomic characterization of the human replisome and replisome-associated factors. In addition to known replisome components, we provide a broad list of proteins that reside in the vicinity of the replisome, some of which were not previously associated with replication. For instance, our data support a link between DNA replication and the Williams-Beuren syndrome and identify ZNF24 as a replication factor. In addition, we reveal that SUMOylation is widespread for factors that concentrate near replisomes, which contrasts with lower UQylation levels at these sites. This resource provides a panoramic view of the proteins that concentrate in the surroundings of the replisome, which should facilitate future investigations on DNA replication and genome maintenance. Cell Rep . 2013 ;3(4):1105-16. 10.1016/j.celrep.2013.03.009 2211-1247 Cell reports 23545495 http://hdl.handle.net/20.500.12105/17682 A proteomic characterization of factors enriched at nascent DNA molecules.