2026-04-29T00:59:45Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/176652024-11-29T20:26:51Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Boskovic, Jasminka
author
Coloma, Javier
author
Aparicio, Tomás
author
Zhou, Min
author
Robinson, Carol V
author
Mendez, Juan
author
Montoya, Guillermo
funder
Fundación Caja Madrid
funder
Ministerio de Educación y Ciencia (España)
2024-02-08T20:23:48Z
2024-02-08T20:23:48Z
2007-07
EMBO Rep . 2007 ;8(7):678-84.
1469-221X
http://hdl.handle.net/20.500.12105/17665
17557111
10.1038/sj.embor.7401002
EMBO reports
Chromosomal DNA replication is strictly regulated through a sequence of steps that involve many macromolecular protein complexes. One of these is the GINS complex, which is required for initiation and elongation phases in eukaryotic DNA replication. The GINS complex consists of four paralogous subunits. At the G1/S transition, GINS is recruited to the origins of replication where it assembles with cell-division cycle protein (Cdc)45 and the minichromosome maintenance mutant (MCM)2-7 to form the Cdc45/Mcm2-7/GINS (CMG) complex, the presumed replicative helicase. We isolated the human GINS complex and have shown that it can bind to DNA. By using single-particle electron microscopy and three-dimensional reconstruction, we obtained a medium-resolution volume of the human GINS complex, which shows a horseshoe shape. Analysis of the protein interactions using mass spectrometry and monoclonal antibody mapping shows the subunit organization within the GINS complex. The structure and DNA-binding data suggest how GINS could interact with DNA and also its possible role in the CMG helicase complex.
eng
Molecular architecture of the human GINS complex.
journal article
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URL
https://repisalud.isciii.es/bitstreams/002b0c62-c3c4-425b-951a-e44473314c0c/download
File
MD5
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application/pdf
moleculararchitectureof_2007.pdf
URL
https://repisalud.isciii.es/bitstreams/9902ec99-f064-48ee-b0b7-71af21248f31/download
File
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text/plain
moleculararchitectureof_2007.pdf.txt