2026-07-21T17:39:40Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/176632024-11-29T17:56:58Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Aparicio, Tomás
author
Guillou, Emmanuelle
author
Coloma, Javier
author
Montoya, Guillermo
author
Méndez, Juan
2024-02-08T20:02:43Z
2024-02-08T20:02:43Z
2009-04
Nucleic Acids Res . 2009;37(7):2087-95
10.1093/nar/gkp065
1362-4962
Nucleic acids research
19223333
http://hdl.handle.net/20.500.12105/17663
The GINS complex, originally discovered in Saccharomyces cerevisiae and Xenopus laevis, binds to DNA replication origins shortly before the onset of S phase and travels with the replication forks after initiation. In this study we present a detailed characterization of the human GINS (hGINS) homolog. Using new antibodies that allow the detection of endogenous hGINS in cells and tissues, we have examined its expression, abundance, subcellular localization and association with other DNA replication proteins. Expression of hGINS is restricted to actively proliferating cells. During the S phase, hGINS becomes part of a Cdc45-MCM-GINS (CMG) complex that is assembled on chromatin. Down-regulation of hGINS destabilizes CMG, causes a G1-S arrest and slows down ongoing DNA replication, effectively blocking cell proliferation. Our data support the notion that hGINS is an essential component of the human replisome.
eng
The human GINS complex associates with Cdc45 and MCM and is essential for DNA replication.
journal article
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URL
https://repisalud.isciii.es/bitstreams/70a3ec39-23f5-4fed-8f3d-9ed61dd32aa2/download
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application/pdf
ThehumanGINScomple_2009.pdf
URL
https://repisalud.isciii.es/bitstreams/fe9fcbfe-6136-44ff-b637-a532de383e5c/download
File
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text/plain
ThehumanGINScomple_2009.pdf.txt