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oai:repisalud.isciii.es:20.500.12105/176632024-11-29T17:56:58Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Aparicio, Tomás author Guillou, Emmanuelle author Coloma, Javier author Montoya, Guillermo author Méndez, Juan author 2009-04 The GINS complex, originally discovered in Saccharomyces cerevisiae and Xenopus laevis, binds to DNA replication origins shortly before the onset of S phase and travels with the replication forks after initiation. In this study we present a detailed characterization of the human GINS (hGINS) homolog. Using new antibodies that allow the detection of endogenous hGINS in cells and tissues, we have examined its expression, abundance, subcellular localization and association with other DNA replication proteins. Expression of hGINS is restricted to actively proliferating cells. During the S phase, hGINS becomes part of a Cdc45-MCM-GINS (CMG) complex that is assembled on chromatin. Down-regulation of hGINS destabilizes CMG, causes a G1-S arrest and slows down ongoing DNA replication, effectively blocking cell proliferation. Our data support the notion that hGINS is an essential component of the human replisome. Nucleic Acids Res . 2009;37(7):2087-95 10.1093/nar/gkp065 1362-4962 Nucleic acids research 19223333 http://hdl.handle.net/20.500.12105/17663 The human GINS complex associates with Cdc45 and MCM and is essential for DNA replication.