2026-06-30T04:57:34Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/176602024-11-29T18:41:56Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Guillou, Emmanuelle
author
Ibarra, Arkaitz
author
Coulon, Vincent
author
Casado-Vela, Juan
author
Rico, Daniel
author
Casal, Ignacio
author
Schwob, Etienne
author
Losada, Ana
author
Mendez, Juan
funder
Ministerio de Ciencia e Innovación (España)
funder
French National Cancer Institute
funder
Fundación Caja Madrid
funder
Fondation pour la recherche médicale (Francia)
funder
Basque Government (España)
2024-02-08T19:23:24Z
2024-02-08T19:23:24Z
2010-12-15
Genes Dev . 2010 ;24(24):2812-22
10.1101/gad.608210
1549-5477
Genes & development
21159821
http://hdl.handle.net/20.500.12105/17660
Genomic DNA is packed in chromatin fibers organized in higher-order structures within the interphase nucleus. One level of organization involves the formation of chromatin loops that may provide a favorable environment to processes such as DNA replication, transcription, and repair. However, little is known about the mechanistic basis of this structuration. Here we demonstrate that cohesin participates in the spatial organization of DNA replication factories in human cells. Cohesin is enriched at replication origins and interacts with prereplication complex proteins. Down-regulation of cohesin slows down S-phase progression by limiting the number of active origins and increasing the length of chromatin loops that correspond with replicon units. These results give a new dimension to the role of cohesin in the architectural organization of interphase chromatin, by showing its participation in DNA replication.
eng
Cohesin organizes chromatin loops at DNA replication factories.
journal article
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URL
https://repisalud.isciii.es/bitstreams/80b3d6a3-2ac9-4bc3-9cde-0ed584a7ad82/download
File
MD5
9542335a4dd1e90baffb1f5552911670
1191522
application/pdf
Cohesinorganizeschromatin_2010.pdf
URL
https://repisalud.isciii.es/bitstreams/9cd91bf3-7e08-4f08-961a-9ded171c30ab/download
File
MD5
b2e89a44b10286ee1a345c8fcaba6ed5
59694
text/plain
Cohesinorganizeschromatin_2010.pdf.txt