2026-06-14T03:45:50Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/176542024-11-29T14:07:47Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Alonso-Gil, Dácil
author
Cuadrado, Ana
author
Giménez-Llorente, Daniel
author
Rodríguez-Corsino, Miriam
author
Losada, Ana
funder
Ministerio de Ciencia e Innovación (España)
funder
Asociación Española Contra el Cáncer
2024-02-08T17:12:50Z
2024-02-08T17:12:50Z
2023-03-10
Nat Commun . 2023;14(1):1326
10.1038/s41467-023-36900-7
2041-1723
Nature communications
36898992
http://hdl.handle.net/20.500.12105/17654
Cohesin organizes the genome through the formation of chromatin loops. NIPBL activates cohesin's ATPase and is essential for loop extrusion, but its requirement for cohesin loading is unclear. Here we have examined the effect of reducing NIPBL levels on the behavior of the two cohesin variants carrying STAG1 or STAG2 by combining a flow cytometry assay to measure chromatin-bound cohesin with analyses of its genome-wide distribution and genome contacts. We show that NIPBL depletion results in increased cohesin-STAG1 on chromatin that further accumulates at CTCF positions while cohesin-STAG2 diminishes genome-wide. Our data are consistent with a model in which NIPBL may not be required for chromatin association of cohesin but it is for loop extrusion, which in turn facilitates stabilization of cohesin-STAG2 at CTCF positions after being loaded elsewhere. In contrast, cohesin-STAG1 binds chromatin and becomes stabilized at CTCF sites even under low NIPBL levels, but genome folding is severely impaired.
eng
Different NIPBL requirements of cohesin-STAG1 and cohesin-STAG2.
journal article
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URL
https://repisalud.isciii.es/bitstreams/4e3e76a3-75ce-4897-821d-1d026ddc3d53/download
File
MD5
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application/pdf
DifferentNIPBLrequirements_2023.pdf
URL
https://repisalud.isciii.es/bitstreams/f5114a0c-0486-465e-a564-67fbbf9808a8/download
File
MD5
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text/plain
DifferentNIPBLrequirements_2023.pdf.txt