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oai:repisalud.isciii.es:20.500.12105/176522024-09-21T10:53:53Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Ayoub, Nabieh author Jeyasekharan, Anand D author Bernal, Juan A author Venkitaraman, Ashok R author 2009-05-15 The dynamics of chromatin-associated proteins control the accessibility of DNA to essential biological transactions like transcription, replication, recombination and repair. Here, we briefly outline what is known about the chromatin changes that occur during the cellular response to DNA breakage, focusing on our recent findings revealing that the chromatin factor HP1beta is mobilized within seconds after DNA damage by an unrecognized signaling cascade mediated by casein kinase 2 (CK2) phosphorylation, paving the way for histone H2AX phosphorylation. We also show here that HP1beta mobilization is neither associated with histone H3 modification on Ser10, an alteration proposed to assist in HP1 ejection from chromatin, nor with evidence of a physical interaction between HP1beta and the CK2 regulatory subunit. Interestingly, following its rapid mobilization, we find that HP1beta gradually re-accumulates on damaged chromatin over a longer time period, suggesting that temporal changes in HP1beta dynamics and interaction with chromatin may assist in different stages of the cellular response to DNA breakage. Cell Cycle. 2009 May 15;8(10):1494-500. http://hdl.handle.net/20.500.12105/17652 19377276 10.4161/cc.8.10.8501 1551-4005 Cell cycle (Georgetown, Tex.) Paving the way for H2AX phosphorylation: chromatin changes in the DNA damage response.