2026-05-22T00:56:27Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/175902024-11-28T14:48:00Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927

00925njm 22002777a 4500 dc Molina, Noemi author Martin-Serrano, Angela author Fernandez, Tahia D. author Tesfaye, Amene author Najera, Francisco author Torres, María J. author Mayorga, Cristobalina author Vida, Yolanda author Montañez, Maria I. author Perez-Inestrosa, Ezequiel author 2018-04-24 Dendrimeric Antigens (DeAns) consist of dendrimers decorated with multiple units of drug antigenic determinants. These conjugates have been shown to be a powerful tool for diagnosing penicillin allergy using in vitro immunoassays, in which they are recognized by specific IgE from allergic patients. Here we propose a new diagnostic approach using DeAns in cellular tests, in which recognition occurs through IgE bound to the basophil surface. Both IgE molecular recognition and subsequent cell activation may be influenced by the tridimensional architecture and size of the immunogens. Structural features of benzylpenicilloyl-DeAn and amoxicilloyl-DeAn (G2 and G4 PAMAM) were studied by diffusion Nuclear Magnetic Resonance (NMR) experiments and are discussed in relation to molecular dynamics simulation (MDS) observations. IgE recognition was clinically evaluated using the basophil activation test (BAT) for allergic patients and tolerant subjects. Diffusion NMR experiments, MDS and cellular studies provide evidence that the size of the DeAn, its antigen composition and tridimensional distribution play key roles in IgE-antigen recognition at the effector cell surface. These results indicate that the fourth generation DeAns induce a higher level of basophil activation in allergic patients. This approach can be considered as a potential complementary diagnostic method for evaluating penicillin allergy. 10.3390/molecules23050997 1420-3049 Molecules http://hdl.handle.net/10668/3344 29695102 http://hdl.handle.net/20.500.12105/17590 PAMAM Dendrimeric antigens Penicillin Drug allergy Basophil activation tests Penicilinas Hipersensibilidad a las drogas Prueba de desgranulación de los Basófilos Dendrimeric Antigens for Drug Allergy Diagnosis: A New Approach for Basophil Activation Tests