2026-08-29T10:23:26Zhttps://repisalud.isciii.es/rest/oai/request
oai:repisalud.isciii.es:20.500.12105/175772024-09-21T22:23:14Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud author Castellano-Castillo, Daniel author Denechaud, Pierre-Damien author Moreno-Indias, Isabel author Tinahones, Francisco author Fajas, Lluis author Queipo-Ortuño, María Isabel author Cardona, Fernando 2024-02-08T14:41:03Z 2024-02-08T14:41:03Z 2018-02-14 10.1371/journal.pone.0192314 1932-6203 PloS one http://hdl.handle.net/10668/12137 29444131 http://hdl.handle.net/20.500.12105/17577 Chromatin immunoprecipitation (ChIP) has gained importance to identify links between the genome and the proteome. Adipose tissue has emerged as an active tissue, which secretes a wide range of molecules that have been related to metabolic and obesity-related disorders, such as diabetes, cardiovascular failure, metabolic syndrome, or cancer. In turn, epigenetics has raised the importance in discerning the possible relationship between metabolic disorders, lifestyle and environment. However, ChIP application in human adipose tissue is limited by several factors, such as sample size, frozen sample availability, high lipid content and cellular composition of the tissue. Here, we optimize the standard protocol of ChIP for small pieces of frozen human adipose tissue. In addition, we test ChIP for the histone mark H3K4m3, which is related to active promoters, and validate the performance of the ChIP by analyzing gene promoters for factors usually studied in adipose tissue using qPCR. Our improvements result in a higher performance in chromatin shearing and DNA recovery of adipocytes from the tissue, which may be useful for ChIP-qPCR or ChIP-seq analysis. eng Chromatin immunoprecipitation improvements for the processing of small frozen pieces of adipose tissue. research article