2026-06-14T03:38:02Zhttps://repisalud.isciii.es/rest/oai/request
oai:repisalud.isciii.es:20.500.12105/175712024-09-21T20:30:25Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927
Repisalud author García-León, Juan Antonio author Kumar, Manoj author Boon, Ruben author Chau, David author One, Jennifer author Wolfs, Esther author Eggermont, Kristel author Berckmans, Pieter author Gunhanlar, Nilhan author de Vrij, Femke author Lendemeijer, Bas author Pavie, Benjamin author Corthout, Nikky author Kushner, Steven A author Dávila, José Carlos author Lambrichts, Ivo author Hu, Wei-Shou author Verfaillie, Catherine M 2024-02-08T14:40:59Z 2024-02-08T14:40:59Z 2018-01-11 10.1016/j.stemcr.2017.12.014 2213-6711 Stem cell reports http://hdl.handle.net/10668/12017 29337119 http://hdl.handle.net/20.500.12105/17571 Scarce access to primary samples and lack of efficient protocols to generate oligodendrocytes (OLs) from human pluripotent stem cells (hPSCs) are hampering our understanding of OL biology and the development of novel therapies. Here, we demonstrate that overexpression of the transcription factor SOX10 is sufficient to generate surface antigen O4-positive (O4+) and myelin basic protein-positive OLs from hPSCs in only 22 days, including from patients with multiple sclerosis or amyotrophic lateral sclerosis. The SOX10-induced O4+ population resembles primary human OLs at the transcriptome level and can myelinate neurons in vivo. Using in vitro OL-neuron co-cultures, myelination of neurons by OLs can also be demonstrated, which can be adapted to a high-throughput screening format to test the response of pro-myelinating drugs. In conclusion, we provide an approach to generate OLs in a very rapid and efficient manner, which can be used for disease modeling, drug discovery efforts, and potentially for therapeutic OL transplantation. eng Amyotrophic lateral sclerosis Disease modeling Drug screening Induced pluripotent stem cells (iPSCs) Multiple sclerosis Myelination Oligodendrocyte SOX10 Single Transcription Factor-Based Fast and Efficient Generation of Oligodendrocytes from Human Pluripotent Stem Cells. research article