2026-04-29T19:34:50Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/175692025-03-24T15:43:37Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927col_20.500.12105_16958col_20.500.12105_16961col_20.500.12105_16977

00925njm 22002777a 4500 dc Benito, José Miguel author Hillung, Julia author Restrepo, Clara author Cuevas, José M author León, Agathe author Ruiz-Mateos, Ezequiel author Palacios-Muñoz, Rosario author Sanjuán, Rafael author 2018-01-01 Background: APOBEC3H (A3H) gene presents variation at 2 positions (rs139297 and rs79323350) leading to a non-functional protein. So far, there is no information on the role played by A3H in spontaneous control of HIV. The aim of this study was to evaluate the A3H polymorphisms distribution in a well-characterized group of Elite Controller (EC) subjects. Methods: We analyzed the genotype distribution of two different SNPs (rs139297 and rs79323350) of A3H in 30 EC patients and compared with 11 non-controller (NC) HIV patients. Genotyping was performed by PCR, cloning and Sanger sequencing. Both polymorphisms were analyzed jointly in order to adequately attribute the active or inactive status of A3H protein. Results: EC subjects included in this study were able to maintain a long-term sustained spontaneous HIV-viral control and optimal CD4-T-cell counts; however, haplotypes leading to an active protein were very poorly represented in these patients. We found that the majority of EC subjects (23/30; 77%) presented allelic combinations leading to an inactive A3H protein, a frequency slightly lower than that observed for NC studied patients (10/11; 91%). Conclusions: The high prevalence of non-functional protein coding-genotypes in EC subjects seems to indicate that other innate restriction factors different from APOBEC3H could be implicated in the replication control exhibited by these subjects. 10.7150/ijms.22317 1449-1907 International journal of medical sciences http://hdl.handle.net/10668/12011 29333092 http://hdl.handle.net/20.500.12105/17569 APOBEC3H polymorphisms HIV Elite controllers rs139297 rs79323350 Role of APOBEC3H in the Viral Control of HIV Elite Controller Patients.