2026-06-14T05:06:46Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/175622024-11-29T20:30:52Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Torres-Ruiz, Raul
author
Grazioso, Tatiana P
author
Brandt, Marta
author
Martinez-Lage, Marta
author
Rodriguez Perales, Sandra
author
Djouder, Nabil
funder
Ministerio de Ciencia e Innovación (España)
funder
Instituto de Salud Carlos III
funder
Asociación Española Contra el Cáncer
2024-02-08T14:31:48Z
2024-02-08T14:31:48Z
2021-09-17
STAR Protoc . 2021;2(3):100631.
10.1016/j.xpro.2021.100631
2666-1667
STAR protocols
34258592
http://hdl.handle.net/20.500.12105/17562
Chromosomal instability (CIN), a type of genomic instability, favors changes in chromosome number and structure and it is associated with the progression and initiation of multiple diseases, including cancer. Therefore, CIN identification and analysis represents a useful tool for cancer diagnosis and treatment. Here, we report an optimized molecular cytogenetic protocol to detect CIN in formalin-fixed, paraffin-embedded mouse and human tissues, using fluorescent in situ hybridization to visualize and quantify chromosomal alterations such as amplifications, deletions, and translocations. For complete information on the generation and use of this protocol, please refer to Brandt et al. (2018).
eng
Detection of chromosome instability by interphase FISH in mouse and human tissues.
journal article
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URL
https://repisalud.isciii.es/bitstreams/3f89c0af-7972-4eee-a29e-a9a91e25912e/download
File
MD5
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application/pdf
detectionofchromosome_2021.pdf
URL
https://repisalud.isciii.es/bitstreams/b404e3a5-dfb6-4d2a-ad61-0b76cf78d3d6/download
File
MD5
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text/plain
detectionofchromosome_2021.pdf.txt