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oai:repisalud.isciii.es:20.500.12105/175622024-11-29T20:30:52Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Torres-Ruiz, Raul author Grazioso, Tatiana P author Brandt, Marta author Martinez-Lage, Marta author Rodriguez Perales, Sandra author Djouder, Nabil author 2021-09-17 Chromosomal instability (CIN), a type of genomic instability, favors changes in chromosome number and structure and it is associated with the progression and initiation of multiple diseases, including cancer. Therefore, CIN identification and analysis represents a useful tool for cancer diagnosis and treatment. Here, we report an optimized molecular cytogenetic protocol to detect CIN in formalin-fixed, paraffin-embedded mouse and human tissues, using fluorescent in situ hybridization to visualize and quantify chromosomal alterations such as amplifications, deletions, and translocations. For complete information on the generation and use of this protocol, please refer to Brandt et al. (2018). STAR Protoc . 2021;2(3):100631. 10.1016/j.xpro.2021.100631 2666-1667 STAR protocols 34258592 http://hdl.handle.net/20.500.12105/17562 Detection of chromosome instability by interphase FISH in mouse and human tissues.