2026-06-14T03:44:09Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/175222025-05-13T11:16:18Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Rodríguez-Antona, C
author
Donato, M T
author
Pareja, E
author
Gómez-Lechón, M J
author
Castell, J V
2024-02-06T12:12:11Z
2024-02-06T12:12:11Z
2001-09-15
Arch Biochem Biophys . 2001;393(2):308-15
10.1006/abbi.2001.2499
0003-9861
Archives of biochemistry and biophysics
11556818
http://hdl.handle.net/20.500.12105/17522
CYP activity and protein contents have been measured in human liver using different techniques. In contrast, CYP mRNA data are scarce and the relationships between CYP mRNA contents and activities have not been established. These studies deserve further attention because mRNA determinations by RT-PCR require a very small amount of material (e.g., liver needle biopsy) and could provide important data regarding CYP expression regulation. In this study we measured in 12 human liver samples the mRNA contents of 10 CYPs by quantitative RT-PCR and the metabolic activities using specific substrates. mRNA contents and activities showed high correlation coefficients for CYP1A1, CYP1A2, CYP3A4, CYP2D6, and CYP2B6 (0.96, 0.94, 0.69, 0.61, and 0.52, respectively), but no significant correlations were found for CYP2C9, CYP2A6, and CYP2E1. The results suggest that the regulation of CYP1A1, CYP1A2, CYP3A4, CYP2D6, and CYP2B6 expression is essentially pretranslational and that their mRNA levels could allow a good estimate of their activity.
eng
Cytochrome P-450 mRNA expression in human liver and its relationship with enzyme activity.
preprint
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T1_3 version aceptada.pdf
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CytochromeP-450mRNAexpressioninhuman_2001.pdf.txt
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T1_3 version aceptada.docx.txt