2026-05-22T02:50:11Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/174852024-09-27T19:35:22Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Herfst, Sander author Mas-Lloret, Vicente author Ver, Lorena S author Wierda, Rutger J author Osterhaus, Albert D M E author Fouchier, Ron A M author Melero, Jose Antonio author 2008-09 Membrane fusion promoted by human metapneumovirus (HMPV) fusion (F) protein was suggested to require low pH (R. M. Schowalter, S. E. Smith, and R. E. Dutch, J. Virol. 80:10931-10941, 2006). Using prototype F proteins representing the four HMPV genetic lineages, we detected low-pH-dependent fusion only with some lineage A proteins and not with lineage B proteins. A glycine at position 294 was found responsible for the low-pH requirement in lineage A proteins. Only 6% of all HMPV lineage A F sequences have 294G, and none of the lineage B sequences have 294G. Thus, acidic pH is not a general trigger of HMPV F proteins for activity. J Virol. 2008 Sep;82(17):8891-5. 10.1128/JVI.00472-08 1098-5514 Journal of virology https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2519679/pdf/0472-08.pdf 18596097 http://hdl.handle.net/20.500.12105/17485 Low-pH-induced membrane fusion mediated by human metapneumovirus F protein is a rare, strain-dependent phenomenon