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oai:repisalud.isciii.es:20.500.12105/174762025-04-24T17:04:42Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Fortún, J author Buitrago, Maria Jose author Gioia, F author Gómez-Gª de la Pedrosa, E author Alvarez, M E author Martín-Dávila, Pilar author Pintado, V author Cobeta, P author Martinez-Castro, N author Soriano, C author Moreno, I author Corral, S author Muñoz, P author Moreno-Jimenez, G author Cuenca-Estrella, Manuel author Moreno-Guillen, S author 2020-08-01 Multiplex quantitative real-time PCR (MRT-PCR) using blood can improve the diagnosis of intra-abdominal candidiasis (IAC). We prospectively studied 39 patients with suspected IAC in the absence of previous antifungal therapy. Blood cultures, MRT-PCR, and β-D-glucan (BDG) in serum were performed in all patients. IAC was defined according to the 2013 European Consensus criteria. For MRT-PCR, the probes targeted the ITS1 or ITS2 regions of ribosomal DNA. Candidaemia was confirmed only in four patients (10%), and IAC criteria were present in 17 patients (43.6%). The sensitivity of MRT-PCR was 25% but increased to 63.6% (P = .06) in plasma obtained prior to volume overload and transfusion; specificity was above 85% in all cases. BDG performance was improved using a cutoff > 260 pg/ml, and improvement was not observed in samples obtained before transfusion. In this cohort of high risk of IAC and low rate of bloodstream infection, the performance of non-culture-based methods (MRT-PCR or BDG) was moderate but may be a complementary tool given the limitations of diagnostic methods available in clinical practice. Volume overload requirements, in combination with other factors, decrease the accuracy of MRT-PCR in patients with IAC. Med Mycol. 2020 Aug 1;58(6):789-796. 10.1093/mmy/myz123 1460-2709 Medical mycology 31811285 http://hdl.handle.net/20.500.12105/17476 Candida spp Multiplex PCR Peritonitis Roles of the multiplex real-time PCR assay and β-D-glucan in a high-risk population for intra-abdominal candidiasis (IAC)