2026-07-21T11:35:04Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/174592024-09-27T19:01:15Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19611col_20.500.12105_19609
Repisalud
author
Morales, Miguel Ángel
author
Cruz, Israel
author
Rubio Muñoz, Jose Miguel
author
Chicharro, Carmen
author
Cañavate, Carmen
author
Laguna, Fernando
author
Alvar, Jorge
funder
Esteve (España)
funder
World Health Organization (WHO/OMS)
funder
Fundación para la Investigación y la Prevención del Sida en España
funder
Instituto de Salud Carlos III
2024-02-05T10:37:25Z
2024-02-05T10:37:25Z
2002-05-15
J Infect Dis. 2002 May 15;185(10):1533-7.
10.1086/340219
0022-1899
The Journal of infectious diseases
11992294
http://hdl.handle.net/20.500.12105/17459
In the Mediterranean basin, Leishmania infantum is a major opportunistic parasite in people with acquired immunodeficiency syndrome (AIDS), and up to 9% of the patients with AIDS suffer from newly acquired or reactivated visceral leishmaniasis. Distinguishing between reinfections and relapses in these patients is important because some apparent treatment failures occur in patients with new rather than reactivated infections. Isoenzyme characterization is limited for use in determining relapsed versus newly acquired leishmaniasis in human immunodeficiency virus (HIV)-infected patients because of the variability of L. infantum and the predominance of the MON-1 zymodeme in people coinfected with HIV. A seminested polymerase chain reaction (PCR) was used to amplify L. infantum minicircle kinetoplast DNA, and, after digestion, the restriction fragment-length polymorphism (RFLP) profiles showed that 3 (7.5%) of 40 patients coinfected with L. infantum and HIV had a new infection, whereas isoenzyme characterization indicated that all 40 patients had infection relapses. These results suggest the utility of this PCR-RFLP analysis in detecting leishmaniasis reinfection in HIV-positive patients.
eng
Relapses versus reinfections in patients coinfected with Leishmania infantum and human immunodeficiency virus type 1
research article
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