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oai:repisalud.isciii.es:20.500.12105/174582024-09-27T21:50:23Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Cruz, Israel author Cañavate, Carmen author Rubio Muñoz, Jose Miguel author Morales, Miguel Ángel author Chicharro, Carmen author Laguna, F author Jiménez-Mejías, M author Sirera, G author Videla, S author Alvar, Jorge author Spanish HIV-Leishmania Study Group author 2002-04 We investigated a Leishmania-specific nested polymerase chain reaction (Ln-PCR) for the diagnosis and treatment monitoring of L. infantum infections in patients co-infected with human immunodeficiency virus (HIV). Peripheral blood and bone marrow samples from 89 HIV patients in Spain suspected of having leishmaniasis were examined by different diagnostic techniques (Ln-PCR, microscopy, NNN culture and indirect fluorescent antibody test). The sensitivity of Ln-PCR compared with microscopy and culture of bone marrow was 95.45% using blood and 100% when using bone marrow. 38 of these patients with confirmed leishmaniasis were entered in a chemotherapy trial (reported elsewhere), and samples from them were collected before treatment, one month after treatment ended and during follow-up (1-20 months), and examined similarly. Ln-PCR was shown to be a good method for testing efficacy of treatment and for predicting relapses after treatment (relapses were predicted on average 5 months earlier than when using classical diagnostic techniques). We suggest that Ln-PCR (especially using peripheral blood) should be the technique of choice for diagnosis, monitoring the success of treatment, and predicting relapses in patients with HIV and suspected or confirmed L. infantum infection. Trans R Soc Trop Med Hyg. 2002 Apr:96 Suppl 1:S185-9. 0035-9203 http://hdl.handle.net/20.500.12105/17458 12055836 10.1016/s0035-9203(02)90074-x Transactions of the Royal Society of Tropical Medicine and Hygiene Leishmaniasis Leishmaniu infantum Human immunodeticiency virus Polymerase chain reaction Diagnosis Follow-up Spain A nested polymerase chain reaction (Ln-PCR) for diagnosing and monitoring Leishmania infantum infection in patients co-infected with human immunodeficiency virus