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00925njm 22002777a 4500 dc Cruz, Israel author Morales, Miguel Ángel author Noguer, I author Rodríguez, A author Alvar, Jorge author 2002-03-30 Needle sharing by intravenous drug users (IVDUs) has been proposed as providing an alternative, artificial, and anthroponotic cycle for leishmania transmission. We looked for parasites in syringes discarded by IVDUs using two different PCR techniques. Leishmania spp were detected in 65 (52%) of 125 syringes collected in southern Madrid, Spain, in 1998, and in 52 (34%) of 154 collected in southwestern Madrid in 2000-01. We found shared restriction fragment length polymorphisms in 12 of 65 positive samples tested, suggesting that syringe sharing can indeed promote the spread of leishmania clones among IVDUs. Lancet. 2002 Mar 30;359(9312):1124-5. 10.1016/s0140-6736(02)08160-6 0140-6736 Lancet (London, England) 11943264 http://hdl.handle.net/20.500.12105/17457 Leishmania in discarded syringes from intravenous drug users