2026-06-14T03:40:33Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/174432024-09-27T23:34:37Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Arenas, Miguel
author
Lorenzo-Redondo, Ramon
author
Lopez-Galindez, Luis Cecilio
funder
Ministerio de Ciencia e Innovación (España)
funder
Instituto de Salud Carlos III
funder
Plan Nacional de I+D+i (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Fundação para a Ciência e Tecnologia (Portugal)
2024-02-02T19:45:09Z
2024-02-02T19:45:09Z
2016-01
Mol Phylogenet Evol. 2016 Jan;94(Pt A):264-70.
10.1016/j.ympev.2015.09.001
1095-9513
Molecular phylogenetics and evolution
26358613
http://hdl.handle.net/20.500.12105/17443
The understanding of the evolutionary processes underlying HIV-1 fitness recovery is fundamental for HIV-1 pathogenesis, antiretroviral treatment and vaccine design. It is known that HIV-1 can present very high mutation and recombination rates, however the specific contribution of these evolutionary forces in the "in vitro" viral fitness recovery has not been simultaneously quantified. To this aim, we analyzed substitution, recombination and molecular adaptation rates in a variety of HIV-1 biological clones derived from a viral isolate after severe population bottlenecks and a number of large population cell culture passages. These clones presented an overall but uneven fitness gain, mean of 3-fold, respect to the initial passage values. We found a significant relationship between the fitness increase and the appearance and fixation of mutations. In addition, these fixed mutations presented molecular signatures of positive selection through the accumulation of non-synonymous substitutions. Interestingly, viral recombination correlated with fitness recovery in most of studied viral quasispecies. The genetic diversity generated by these evolutionary processes was positively correlated with the viral fitness. We conclude that HIV-1 fitness recovery can be derived from the genetic heterogeneity generated through both mutation and recombination, and under diversifying molecular adaptation. The findings also suggest nonrandom evolutionary pathways for in vitro fitness recovery.
eng
HIV-1 molecular evolution
Mutation
Viral recombination
Molecular 40 adaptation
Genetic heterogeneity
Fitness recovery
Influence of mutation and recombination on HIV-1 in vitro fitness recovery
research article
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URL
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InfluenceMutationRecombinationHIV-1_2016.pdf
URL
https://repisalud.isciii.es/bitstreams/07552fb1-0a89-4252-80d2-a6925dd06433/download
File
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InfluenceMutationRecombinationHIV-1_2016.pdf.txt