2026-06-14T03:44:13Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/174412024-11-28T18:18:04Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16962

00925njm 22002777a 4500 dc Escolano-Martinez, Maria S author Domenech, Arnau author Yuste, Jose Enrique author Cercenado, Maria I author Ardanuy, Carmen author Liñares, Josefina author de la Campa, Adela G author Martin-Galiano, Antonio Javier author 2016-07 Objectives: Many outer multidomain proteins play fundamental virulent roles in an allele-dependent manner. We aimed to investigate the influence of the outer SP1992 protein, here renamed DiiA (Dimorphic invasion-involved A), in pneumococcal disease. Methods: The presence and type of diiA allele was screened by PCR in 560 clinical isolates. Isogenic mutants carrying progressive diiA deletions were constructed and checked in mouse models of infection. DiiA binding to human molecules was carried out by surface plasmon resonance. Results: The diiA gene is exclusive of Streptococcus pneumoniae and included in the core genome. DiiA variants contain one or two imperfect repeats (R1 and R2), an unstructured region and a cell-wall anchor domain. Clonal complexes carrying both repeats were associated with invasive disease, while those carrying R2 preferentially caused non-invasive syndromes in patients with underlying risk factors. Mutants lacking both repeats were less efficient in nasopharyngeal colonization and dissemination from lungs. Moreover, the ΔdiiA defective strain suffered a severe impairment in bacterial proliferation in blood. Purified DiiA bound to collagen and lactoferrin with high affinity. Conclusions: DiiA is a distinctive pneumococcal virulence factor contributing to colonization and long-term invasion in this pathogen. J Infect. 2016 Jul;73(1):71-81. 10.1016/j.jinf.2016.04.010 1532-2742 The Journal of infection 27105656 http://hdl.handle.net/20.500.12105/17441 Adhesine Genomics Hypothetical protein Invasion Vaccine Virulence factor DiiA is a novel dimorphic cell wall protein of Streptococcus pneumoniae involved in invasive disease