2026-06-14T05:05:10Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/174392024-09-27T22:03:13Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Oliva, María A
author
Martin-Galiano, Antonio Javier
author
Sakaguchi, Yoshihiko
author
Andreu, José M
2024-02-02T17:21:15Z
2024-02-02T17:21:15Z
2012-05-15
Proc Natl Acad Sci USA. 2012 May 15;109(20):7711-6.
10.1073/pnas.1121546109
1091-6490
Proceedings of the National Academy of Sciences of the United States of America
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356609/pdf/pnas.201121546.pdf
22538818
http://hdl.handle.net/20.500.12105/17439
Partition systems are responsible for the process whereby large and essential plasmids are accurately positioned to daughter cells during bacterial division. They are typically made of three components: a centromere-like DNA zone, an adaptor protein, and an assembling protein that is either a Walker-box ATPase (type I) or an actin-like ATPase (type II). A recently described type III segregation system has a tubulin/FtsZ-like protein, called TubZ, for plasmid movement. Here, we present the 2.3 Å structure and dynamic assembly of a TubZ tubulin homolog from a bacteriophage and unravel the Clostridium botulinum phage c-st type III partition system. Using biochemical and biophysical approaches, we prove that a gene upstream from tubZ encodes the partner TubR and localize the centromeric region (tubS), both of which are essential for anchoring phage DNA to the motile TubZ filaments. Finally, we describe a conserved fourth component, TubY, which modulates the TubZ-R-S complex interaction.
eng
DNA segregation
Cytomotive filaments
Virulence
Plasmid partitioning
Tubulin homolog TubZ in a phage-encoded partition system
research article
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