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oai:repisalud.isciii.es:20.500.12105/174392024-09-27T22:03:13Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Oliva, María A author Martin-Galiano, Antonio Javier author Sakaguchi, Yoshihiko author Andreu, José M author 2012-05-15 Partition systems are responsible for the process whereby large and essential plasmids are accurately positioned to daughter cells during bacterial division. They are typically made of three components: a centromere-like DNA zone, an adaptor protein, and an assembling protein that is either a Walker-box ATPase (type I) or an actin-like ATPase (type II). A recently described type III segregation system has a tubulin/FtsZ-like protein, called TubZ, for plasmid movement. Here, we present the 2.3 Å structure and dynamic assembly of a TubZ tubulin homolog from a bacteriophage and unravel the Clostridium botulinum phage c-st type III partition system. Using biochemical and biophysical approaches, we prove that a gene upstream from tubZ encodes the partner TubR and localize the centromeric region (tubS), both of which are essential for anchoring phage DNA to the motile TubZ filaments. Finally, we describe a conserved fourth component, TubY, which modulates the TubZ-R-S complex interaction. Proc Natl Acad Sci USA. 2012 May 15;109(20):7711-6. 10.1073/pnas.1121546109 1091-6490 Proceedings of the National Academy of Sciences of the United States of America https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356609/pdf/pnas.201121546.pdf 22538818 http://hdl.handle.net/20.500.12105/17439 DNA segregation Cytomotive filaments Virulence Plasmid partitioning Tubulin homolog TubZ in a phage-encoded partition system