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oai:repisalud.isciii.es:20.500.12105/174142024-11-29T18:05:00Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Diéguez-Hurtado, Rodrigo author Martín, Javier author Martínez-Corral, Inés author Martinez Garcia, Maria Dolores author Megías, Diego author Olmeda, David author Ortega Jimenez, Sagrario author 2011-01 Cre/loxP-dependent expression of fluorescent proteins represents a powerful biological tool for cell lineage, fate-mapping, and genetic analysis. Live tissue imaging has significantly improved with the development of far-red fluorescent proteins, with optimized spectral characteristics for in vivo applications. Here, we report the generation of the first transgenic mouse line expressing the far-red fluorescent protein Katushka, driven by the hybrid CAG promoter upon Cre-mediated recombination. After germ line or tissue-specific Cre-driven reporter activation, Katushka expression is strong and ubiquitous, without toxic effects, allowing fluorescence detection in fresh and fixed samples from all tissues examined. Moreover, fluorescence can be detected by in vivo noninvasive whole-body imaging when Katuhska is expressed exclusively in a specific cell population deep within the animal body such as pancreatic beta cells. Thus, this reporter model enables early, widespread, and sensitive in vivo detection of Cre activity and should provide a versatile tool for a wide spectrum of fluorescence and live-imaging applications. Genesis . 2011;49(1):36-45. 10.1002/dvg.20685 1526-968X Genesis (New York, N.Y. : 2000) 21254335 http://hdl.handle.net/20.500.12105/17414 A Cre-reporter transgenic mouse expressing the far-red fluorescent protein Katushka.