2026-06-14T03:44:19Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/173822025-05-09T09:27:15Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Blázquez‐García, Marina
author
Quinchia, Jennifer
author
Ruiz‐Valdepeñas Montiel, Víctor
author
Torrente-Rodríguez, Rebeca M
author
Serafín, Verónica
author
Garranzo-Asensio, Maria
author
García‐Romero, Ana
author
Orozco, Jahir
author
Barderas Manchado, Rodrigo
author
Pingarrón, José M
author
Campuzano, Susana
funder
Unión Europea. Comisión Europea. H2020
funder
Instituto de Salud Carlos III
funder
Agencia Estatal de Investigación (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
University of Antioquia (Colombia)
funder
Max Planck Society
funder
Ministerio de Ciencia Tecnología e Innovación (Colombia)
2024-01-31T08:09:38Z
2024-01-31T08:09:38Z
2024
Blázquez‐García, Marina; Quinchia, Jennifer; Ruiz‐Valdepeñas Montiel, Víctor; Torrente‐Rodríguez, Rebeca M.; Serafín, Verónica; Garranzo-Asensio, Maria; García‐Romero, Ana; Orozco, Jahir; Barderas Manchado, Rodrigo; Pingarrón, José M.; Campuzano, Susana. Electroanalytical Immunotool to Determine Matricellular Protein Periostin, a Stromal Biomarker of Prognosis in Colorectal Cancer. ChemElectroChem.2024,11:e202300641.
10.1002/celc.202300641
2196-0216
2196-0216
ChemElectroChem
http://hdl.handle.net/20.500.12105/17382
Tumor-associated stroma biomarkers are emerging as key signaling molecules of metastasis in colorectal cancer (CRC). In this sense, periostin (POSTN), a protein of the extracellular matrix from the stromal compartment, is envisioned as a potential stromal prognostic biomarker, which facilitates the application of pertinent treatments. In this work, we report an easy-to-handle amperometric sandwich-based immunosensing strategy for the determination of POSTN involving commercial magnetic microparticles (MBs), disposable carbon electrodes, and the horseradish peroxidase (HRP)/H2O2/hydroquinone (HQ) electrochemical system. The method allowed a dynamic linear range between 0.47 and 25 ngmL-1 and a limit of detection, LOD, of 0.14 ngmL-1 compatible with clinical demands. The method was applied to the analysis of POSTN in a variety of cancer-related real bio-scenarios including cell extracts and secretomes from CRC cells, and plasma from CRC patients at different stages. The potentials of this firstly developed MBsassisted immunoplatform are justified by distinguishing between metastatic abilities of cultured CRC cells through the analysis of their extracts and secretomes, and by differentiating between healthy controls and CRC patients in just 60 min. Therefore, the developed immunoplatform can be envisaged as a novel and profitable tool for exploring the most uncharacterized but prospective tumor areas.
eng
POSTN
Magnetic microparticles
Disposable carbon electrodes
Colorectal cancer
Plasma
Electroanalytical Immunotool to Determine Matricellular Protein Periostin, a Stromal Biomarker of Prognosis in Colorectal Cancer
research article
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
URL
https://repisalud.isciii.es/bitstreams/06e4537a-79dc-4a28-93bc-e2307708a25a/download
File
MD5
684c4d0b673079ba2790964df383eecb
2775232
application/pdf
ElectroanalyticalImmunotoolDetermineMatricellular_2024.pdf
URL
https://repisalud.isciii.es/bitstreams/f1fab858-d0c7-4805-ab3a-730336c6222a/download
File
MD5
c6451be7acdfdbbdf992aaf2e0413600
243907
application/pdf
Supplementary_ElectroanalyticalImmunotoolDetermineMatricellular_2024.pdf
URL
https://repisalud.isciii.es/bitstreams/e4f6d24e-a84a-421d-92b5-53c52d0e6d0d/download
File
MD5
eaa6f8ad5d6bcd6599cbaf337e2df98c
56577
text/plain
ElectroanalyticalImmunotooltoDetermineMatricellular_2023.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/01413c80-3493-48f0-8c7b-eea0363e13f3/download
File
MD5
32e9af4112aab4a287f418cb99d4e868
8180
text/plain
Suplementary_ElectroanalyticalImmunotooltoDetermineMatricellular_2023.pdf.txt