2026-05-22T00:07:55Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/173482024-09-21T18:55:35Zcom_20.500.12105_15322com_20.500.12105_2051col_20.500.12105_16927col_20.500.12105_16982
Repisalud
author
Oliva-Olivera, Wilfredo
author
Moreno-Indias, Isabel
author
Coín-Aragüez, Leticia
author
Lhamyani, Said
author
Alcaide Torres, Juan
author
Fernández-Veledo, Sonia
author
Vendrell, Joan
author
Camargo, Antonio
author
El Bekay, Rajaa
author
Tinahones, Francisco José
authoraffiliation
[Oliva-Olivera, Wilfredo] Univ Malaga UMA, Hosp Malaga Virgen de la Victoria, Dept Clin Endocrinol & Nutr, Inst Biomed Res Malaga IBIMA, Malaga, Spain
authoraffiliation
[Moreno-Indias, Isabel] Univ Malaga UMA, Hosp Malaga Virgen de la Victoria, Dept Clin Endocrinol & Nutr, Inst Biomed Res Malaga IBIMA, Malaga, Spain
authoraffiliation
[Coin-Araguez, Leticia] Univ Malaga UMA, Hosp Malaga Virgen de la Victoria, Dept Clin Endocrinol & Nutr, Inst Biomed Res Malaga IBIMA, Malaga, Spain
authoraffiliation
[Alcaide Torres, Juan] Univ Malaga UMA, Hosp Malaga Virgen de la Victoria, Dept Clin Endocrinol & Nutr, Inst Biomed Res Malaga IBIMA, Malaga, Spain
authoraffiliation
[El Bekay, Rajaa] Univ Malaga UMA, Hosp Malaga Virgen de la Victoria, Dept Clin Endocrinol & Nutr, Inst Biomed Res Malaga IBIMA, Malaga, Spain
authoraffiliation
[Jose Tinahones, Francisco] Univ Malaga UMA, Hosp Malaga Virgen de la Victoria, Dept Clin Endocrinol & Nutr, Inst Biomed Res Malaga IBIMA, Malaga, Spain
authoraffiliation
[Oliva-Olivera, Wilfredo] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nut, Madrid, Spain
authoraffiliation
[Moreno-Indias, Isabel] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nut, Madrid, Spain
authoraffiliation
[Coin-Araguez, Leticia] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nut, Madrid, Spain
authoraffiliation
[Alcaide Torres, Juan] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nut, Madrid, Spain
authoraffiliation
[Camargo, Antonio] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nut, Madrid, Spain
authoraffiliation
[El Bekay, Rajaa] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nut, Madrid, Spain
authoraffiliation
[Jose Tinahones, Francisco] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nut, Madrid, Spain
authoraffiliation
[Lhamyani, Said] Univ Malaga UMA, Res Lab, Sci Sch, Malaga, Spain
authoraffiliation
[Fernandez-Veledo, Sonia] Univ Rovirai Virgili, Hosp Univ Tarragona Joan 23, Inst Invest Sanitaria Pere Virgili, Tarragona, Spain
authoraffiliation
[Vendrell, Joan] Univ Rovirai Virgili, Hosp Univ Tarragona Joan 23, Inst Invest Sanitaria Pere Virgili, Tarragona, Spain
authoraffiliation
[Fernandez-Veledo, Sonia] Inst Salud Carlos III, CIBER Diabet & Enfermedades Metab Asociadas CIBER, Madrid, Spain
authoraffiliation
[Vendrell, Joan] Inst Salud Carlos III, CIBER Diabet & Enfermedades Metab Asociadas CIBER, Madrid, Spain
authoraffiliation
[Camargo, Antonio] Univ Cordoba, Reina Sofia Univ Hosp, Lipids & Atherosclerosis Unit, IMIBIC, Cordoba, Spain
2024-01-23T20:13:20Z
2024-01-23T20:13:20Z
2017-11-22
10.1371/journal.pone.0188324
1932-6203
PloS one
http://hdl.handle.net/10668/11835
29166648
http://hdl.handle.net/20.500.12105/17348
Multiple studies suggest that hypoxia, together with inflammation, could be one of the phenomena involved in the onset and progression of obesity-related insulin resistance. In addition, dysfunction of adipose tissue in obese subjects with metabolic syndrome is associated with decreased angiogenesis. However, some subjects with a high body mass index do not develop metabolic abnormalities associated with obesity. The aim of the current study was to examine the neovascular properties of visceral adipose tissue-derived multipotent mesenchymal cells subjected to hypoxia (hypox-visASCs) from normal-weight subjects (Nw) and obese patients with metabolic syndrome (MS) and without metabolic syndrome (NonMS). This was a 2-year study to enroll subjects who underwent bariatric surgery or cholecystectomy. Eight patients who underwent either bariatric surgery or cholecystectomy (27 patients) participated in the study. Visceral adipose tissue samples from Nw, MS and NonMS subjects were processed by enzymatic digestion. VisASCs cultured under hypoxic conditions were characterized by tubule formation assay, ELISA, flow cytometry, migration rate, and qRT-PCR, and the effects of visASCs-conditioned medium on survival and endothelial cell tubule formation were evaluated. Hypox-visASCs from NonMS subjects showed a greater capacity for tubule formation than hypox-visASCs from Nw and MS subjects. The lower percentage of CD140b+/CD44+ and CD140b+/CD184+ cells observed in hypox-visASCs from NonMS subjects compared to MS subjects was accompanied not only by a lower migration rate from the chemotactic effects of stromal cell derived factor 1α, but also by lower levels of NOX5 mRNA expression. While the levels of monocyte chemoattractant protein 1 mRNA expressed by hypox-visASCs correlated positively with the body mass index and waist circumference of the subjects, the concentration of vascular endothelial growth factor present in hypox-visASC-conditioned culture medium decreased significantly with increasing plasma glucose. The survival rate and tubules formed by endothelial cells cultured in hypox-visASC-conditioned medium decreased significantly with increasing homeostasis model assessment to quantify insulin resistance. Our results suggest that hypox-visASCs from NonMS subjects could promote healthy adipose tissue expansion, while hypox-visASCs from MS subjects appear to contribute to the decreased angiogenic potential and increased inflammation underlying adipose tissue dysfunction in obesity. Our results emphasize the importance of taking into account not only the BMI but also the metabolic profile of the subjects during the implementation of ASCs-based therapy to promote neovascularization.
eng
Improve postnatal neovascularization
Mesenchymal stem-cells
Insulin-resistance
Stromal cells
Macrophage infiltration
Tissue angiogenesis
Endothelial-cells
Oxidative stress
Different response to hypoxia of adipose-derived multipotent cells from obese subjects with and without metabolic syndrome.
research article